Document Detail


Biochemical and enzymatic characterization of two basic Asp49 phospholipase A2 isoforms from Lachesis muta muta (Surucucu) venom.
MedLine Citation:
PMID:  16005152     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Two basic phospholipase A2 (PLA2) isoforms were isolated from Lachesis muta muta snake venom and partially characterized. The venom was fractionated by molecular exclusion chromatography in ammonium bicarbonate buffer followed by reverse-phase HPLC on a C-18 mu-Bondapack column and RP-HPLC on a C-8 column. From liquid chromatography-electrospray ionization/mass spectrometry, the molecular mass of the two isoforms LmTX-I and LmTX-II was respectively measured as 14,245.4 and 14,186.2 Da. The pI was respectively estimated to be 8.7 and 8.6 for LmTX-I and LmTX-II, as determined by two-dimensional electrophoresis. The two proteins were sequenced and differentiated from each other by a single amino acid substitution, Arg65 (LmTX-I)-->Pro65 (LmTX-II). The amino acid sequence showed a high degree of homology between PLA2 isoforms from Lachesis muta muta and other PLA2 snake venoms. LmTX-I and LmTX-II had PLA2 activity in the presence of a synthetic substrate and showed a minimum sigmoidal behaviour; with maximal activity at pH 8.0 and 35-45 degrees C. Full PLA2 activity required Ca2+ and was respectively inhibited by Cu2+ and Zn2+ in the presence and absence of Ca2+. Crotapotin from Crotalus durissus cascavella rattlesnake venom significantly inhibited (P<0.05) the enzymatic activity of LmTX-I, suggesting that the binding site for crotapotin in this PLA2 was similar to another in the basic PLA2 of the crotoxin complex from C. durissus cascavella venom.
Authors:
Daniela C S Damico; Sérgio Lilla; Gilberto de Nucci; Luis A Ponce-Soto; Flávia V Winck; José Camillo Novello; Sérgio Marangoni
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't     Date:  2005-06-17
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  1726     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  2005 Oct 
Date Detail:
Created Date:  2005-10-28     Completed Date:  2006-05-23     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  75-86     Citation Subset:  IM    
Affiliation:
Department of Biochemistry, Institute of Biology, State University of Campinas (UNICAMP), Campinas, SP, Brazil. dcdamico@unicamp.br
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Base Sequence
Chemical Fractionation
Chromatography, Gel
Chromatography, High Pressure Liquid
Crotalid Venoms / enzymology*
Crotoxin / metabolism
Electrophoresis, Gel, Two-Dimensional
Mass Spectrometry
Metals, Heavy / metabolism
Molecular Sequence Data
Phospholipases A / chemistry*,  genetics,  isolation & purification*,  metabolism
Phospholipases A2
Sequence Analysis, DNA
Viperidae*
Chemical
Reg. No./Substance:
0/Crotalid Venoms; 0/Lachesis venom; 0/Metals, Heavy; 9007-40-3/Crotoxin; EC 3.1.1.-/Phospholipases A; EC 3.1.1.4/Phospholipases A2

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