Document Detail

Biochemical and electron microscopic observations of cytotoxic effect of a fraction from hemolytic streptococcus on Ehrlich ascites carcinoma cells.
MedLine Citation:
PMID:  6188869     Owner:  NLM     Status:  MEDLINE    
A protein fraction (60-F), obtained from cell-free extract of living hemolytic streptococcus, Su-strain, by 50-60% saturation with ammonium sulfate, inhibited the de novo synthesis of nucleic acids and proteins in Ehrlich ascites carcinoma (EAC) cells. 60-F released RNA but not DNA from EAC cells. This cytotoxic or cytolytic effect of 60-F was substantiated morphologically by scanning and transmission electron microscopic (SEM and TEM) observations, showing that 60-F induced cellular changes such as a loss of microvilli, bleb formation, cell deformity and partial gap of cell membrane in EAC cells. In addition, it was found that 60-F was more stable in RPMI-1640 medium supplemented with fetal calf serum than in other various media examined and was sensitive to temperature, pH changes and trypsin digestion.
Y Higuchi; S Nakamura; K Itoh; M Yamamura
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Japanese journal of experimental medicine     Volume:  52     ISSN:  0021-5031     ISO Abbreviation:  Jpn. J. Exp. Med.     Publication Date:  1982 Oct 
Date Detail:
Created Date:  1983-06-10     Completed Date:  1983-06-10     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9800765     Medline TA:  Jpn J Exp Med     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  221-30     Citation Subset:  IM    
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MeSH Terms
Amino Acids / metabolism
Bacterial Proteins / pharmacology*
Carcinoma, Ehrlich Tumor / metabolism*,  ultrastructure
Cell Membrane / ultrastructure
Cytotoxins / pharmacology
DNA / metabolism
Drug Stability
Microscopy, Electron
Microvilli / ultrastructure
Nucleosides / metabolism
RNA / metabolism
Streptococcus / analysis*
Reg. No./Substance:
0/Amino Acids; 0/Bacterial Proteins; 0/Cytotoxins; 0/Nucleosides; 63231-63-0/RNA; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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