| Biochemical detection of adenosine deaminase in Trypanosoma evansi. | |
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MedLine Citation:
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PMID: 21420956 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Biochemical and molecular research on parasites has increased considerably in trypanosomes in the recent years. Many of them have the purpose of identify areas, proteins and structures of the parasite which are vulnerable and could be used in therapy against the protozoan. Based on this hypothesis this study aimed to detect biochemically the enzyme adenosine deaminase (ADA) in Trypanosoma evansi, and to adapt an assay to the measurement of its activity in trypomastigotes. Firstly, the parasites were separated from the blood of mice experimentally infected with a DEAE-cellulose column. The ADA activity in trypomastigotes was evaluated at concentrations of 0.1, 0.2, 0.5, 0.6 and 0.8 mg of protein by spectrophotometry. ADA activity was observed in the parasites at all concentrations tested and its activity was proportional to the concentration of protein, ranging between 0.64 and 2.24 U/L in the lowest and highest concentration of protein, respectively. Therefore, it is possible to detect biochemically ADA in T. evansi, an enzyme that may be associated with vital functions of the parasite, similar to what occurs in mammals. This knowledge may be useful in the association of the chemotherapic treatment with specific inhibitors of the enzyme, in future studies. |
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Authors:
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Aleksandro S Da Silva; Victor C Pimentel; Jeandre A S Jaques; Patrícia Wolkmer; Kaio C S Tavares; Cícera R Lazzarotto; Luiz C Miletti; Maria Rosa C Schetinger; Cinthia M Mazzanti; Sonia T A Lopes; Silvia G Monteiro |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-3-18 |
Journal Detail:
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Title: Experimental parasitology Volume: - ISSN: 1090-2449 ISO Abbreviation: - Publication Date: 2011 Mar |
Date Detail:
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Created Date: 2011-3-22 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0370713 Medline TA: Exp Parasitol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2011. Published by Elsevier Inc. |
Affiliation:
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Department of Microbiology and Parasitology, Universidade Federal de Santa Maria, Brazil. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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