Document Detail


Biochemical contribution to diagnosis and study of a new case of D-glyceric acidemia/aciduria.
MedLine Citation:
PMID:  2551543     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
During organic acid screening by gas chromatography/mass spectrometry, we detected a large peak corresponding to glyceric acid in a patient's urine sample. The D(+) configuration was demonstrated by a polarimetric method and by enzymatic stereospecificity of D-glycerate dehydrogenase (EC 1.1.1.29). We biochemically investigated this fifth reported case of D-glyceric acidemia. In our patient, loading tests with L-serine and fructose led to an increase of D-glyceric acid in both plasma and urine. Determination of other metabolites involved in D-glycerate metabolism revealed no abnormality in any sample examined. After comparing all our results with those of the preceding observations described in the literature, we suggest a possible enzymatic defect located on one of the metabolic pathways shared by fructose and L-serine, possibly at the level of hepatic D-glycerate kinase (EC 2.7.1.31). Nevertheless, a primary defect of L-serine catabolism cannot be entirely excluded.
Authors:
M Fontaine; N Porchet; C Largilliere; S Marrakchi; M Lhermitte; J P Aubert; P Degand
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Publication Detail:
Type:  Case Reports; Journal Article; Review    
Journal Detail:
Title:  Clinical chemistry     Volume:  35     ISSN:  0009-9147     ISO Abbreviation:  Clin. Chem.     Publication Date:  1989 Oct 
Date Detail:
Created Date:  1989-11-17     Completed Date:  1989-11-17     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  9421549     Medline TA:  Clin Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2148-51     Citation Subset:  IM    
Affiliation:
Laboratoire de Biochimie, Hôpital Huriez, Centre Hospitalier, Régional et Universitaire, Lille, France.
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MeSH Terms
Descriptor/Qualifier:
Chemical Phenomena
Chemistry
Female
Fructose / diagnostic use
Gas Chromatography-Mass Spectrometry
Glyceric Acids / metabolism,  urine*
Humans
Indicators and Reagents
Infant
Metabolism, Inborn Errors / diagnosis*,  urine
Phosphotransferases / metabolism*
Phosphotransferases (Alcohol Group Acceptor)*
Serine / diagnostic use
Chemical
Reg. No./Substance:
0/Glyceric Acids; 0/Indicators and Reagents; 30237-26-4/Fructose; 473-81-4/glyceric acid; 56-45-1/Serine; EC 2.7.-/Phosphotransferases; EC 2.7.1.-/Phosphotransferases (Alcohol Group Acceptor); EC 2.7.1.31/glycerate kinase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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