Document Detail


Biochemical characterization of rat intestine development using high-resolution magic-angle-spinning 1H NMR spectroscopy and multivariate data analysis.
MedLine Citation:
PMID:  16083283     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We report details of metabolic profiles for small intestinal samples obtained using high-resolution magic-angle-spinning (HRMAS) (1)H NMR spectroscopy. Intact samples of jejunum and ileum from male Long Evans rats were analyzed on a 600 MHz spectrometer using standard one and two-dimensional (1)H NMR spectroscopic pulse sequences. The metabolic profiles of ileum and jejunum predominantly comprised a number of amino acids, lipids, glycerophosphocholine (GPC), choline, creatine, and ethanol, a number of carboxylic acids including acetate and lactate, and nucleoside bases including cytosine, isocytosine, and uracil. Principal component analysis (PCA) was applied to these NMR data to characterize the biochemical differences between jejunum and ileum tissues. Compared with ileum, jejunum contained higher levels of lipids, GPC, choline, lactate and creatinine, but lower levels of amino acids and acetate. In addition, the age dependence of the biochemical composition of intestinal tissues from young rats (15, 36 days and 3-4 months old) was studied. In general, levels of lipids, lactate, taurine and creatinine were positively correlated with age while amino acids and GPC decreased in the older age group. This study will provide a metabolic reference for further studies assessing the metabolic consequences of nutrition, stress and gut microbiota on intestinal composition.
Authors:
Yulan Wang; Huiru Tang; Elaine Holmes; John C Lindon; Marco E Turini; Norbert Sprenger; Gabriela Bergonzelli; Laurent B Fay; Sunil Kochhar; Jeremy K Nicholson
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of proteome research     Volume:  4     ISSN:  1535-3893     ISO Abbreviation:  J. Proteome Res.     Publication Date:    2005 Jul-Aug
Date Detail:
Created Date:  2005-08-08     Completed Date:  2005-10-13     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  101128775     Medline TA:  J Proteome Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1324-9     Citation Subset:  IM    
Affiliation:
Biological Chemistry, Biomedical Sciences Division, Faculty of Medicine, Imperial College London, Sir Alexander Fleming Building, South Kensington, London, SW7, 2AZ, United Kingdom. yulan.wang@imperial.ac.uk
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MeSH Terms
Descriptor/Qualifier:
Age Factors
Animals
Intestine, Small* / chemistry,  growth & development,  metabolism
Male
Multivariate Analysis
Nuclear Magnetic Resonance, Biomolecular / methods*
Protons
Rats
Rats, Long-Evans
Chemical
Reg. No./Substance:
0/Protons

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