Document Detail


Biochemical characterization of a novel ArsA ATPase complex from Alkaliphilus metalliredigens QYMF.
MedLine Citation:
PMID:  20553716     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The two putative ars operons in Alkaliphilus metalliredigens QYMF are distinctive in that the arsA gene is split in halves, amarsA1 and amarsA2, and, acr3 but not an arsB gene coexists with arsA. Heterologous expression of one of the A. metalliredigensars operons (ars1) conferred arsenite but not antimonite resistance to DeltaarsEscherichia coli. Only the co-expressed AmArsA1 and AmArsA2 displayed arsenite or antimonite stimulated ATPase activity. The results show that AmArsA1-AmArsA2 interaction is needed to form the functional ArsA ATPase. This novel AmArsA1-AmArsA2 complex may provide insight in how it participates with Acr3 in arsenite detoxification.
Authors:
Hsueh-Liang Fu; Barry P Rosen; Hiranmoy Bhattacharjee
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2010-05-27
Journal Detail:
Title:  FEBS letters     Volume:  584     ISSN:  1873-3468     ISO Abbreviation:  FEBS Lett.     Publication Date:  2010 Jul 
Date Detail:
Created Date:  2010-07-08     Completed Date:  2010-07-27     Revised Date:  2012-10-09    
Medline Journal Info:
Nlm Unique ID:  0155157     Medline TA:  FEBS Lett     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  3089-94     Citation Subset:  IM    
Copyright Information:
Copyright 2010 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
Affiliation:
Department of Biochemistry and Molecular Biology, Wayne State University, School of Medicine, Detroit, MI 48201, USA.
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MeSH Terms
Descriptor/Qualifier:
Adenosine Triphosphatases / chemistry,  genetics*,  metabolism*
Antimony
Arsenites
Gram-Positive Bacteria / genetics,  metabolism
Operon
Grant Support
ID/Acronym/Agency:
GM55425/GM/NIGMS NIH HHS; R37 GM055425/GM/NIGMS NIH HHS; R37 GM055425-27/GM/NIGMS NIH HHS; R37 GM055425-29/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Arsenites; 15502-74-6/arsenite; 39349-74-1/antimonite; 7440-36-0/Antimony; EC 3.6.1.-/Adenosine Triphosphatases
Comments/Corrections

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