Document Detail


Biochemical characterization of elongase activity in corn (Zea mays L.) roots.
MedLine Citation:
PMID:  15652569     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Chemical analysis of 4-day-old corn (Zea mays L.) root cell walls revealed that the lipophilic biopolymer suberin forms an important constituent of rhizodermal and hypodermal cell walls. Identified aliphatic monomers had chain lengths ranging from C16 to C26 and they belonged to 5 substance classes (omega-hydroxycarboxylic acids, 1,omega-dicarboxylic acids, 2-hydroxycarboxylic acids, carboxylic acids and alcohols) by which suberin is characterized. Biochemical experiments proved the occurrence of elongase activities in corn roots. Highest enzymatic activities were found in corn root microsomes, and major products synthesized by root elongases were elongated fatty acids with chain lengths ranging from C20 to C24. Preferred substrates of root elongases were acyl-CoAs of the chain length C18 and C20, whereas monounsaturated acyl-CoAs (C16:1 and C18:1) and acyl-CoAs of lower (C12-C16) and higher chain lengths (C22-C24) were rarely elongated. Elongase activities significantly decreased over the length (40 cm) of 10-day-old corn roots going from the young tip to the older base of the root. Thus, results presented here show the presence and activity of elongases in roots of plants.
Authors:
Lukas Schreiber; Rochus Franke; René Lessire
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Phytochemistry     Volume:  66     ISSN:  0031-9422     ISO Abbreviation:  Phytochemistry     Publication Date:  2005 Jan 
Date Detail:
Created Date:  2005-01-17     Completed Date:  2005-04-11     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0151434     Medline TA:  Phytochemistry     Country:  United States    
Other Details:
Languages:  eng     Pagination:  131-8     Citation Subset:  IM    
Affiliation:
Department of Ecophysiology, Institute of Cellular and Molecular Botany (IZMB), University of Bonn, Kirschallee 1, D-53115 Bonn, Germany. lukas.schreiber@uni-bonn.de
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MeSH Terms
Descriptor/Qualifier:
Acetyltransferases / metabolism*
Lipids
Membrane Lipids / biosynthesis
Plant Roots / enzymology*,  ultrastructure
Zea mays / enzymology*,  ultrastructure
Chemical
Reg. No./Substance:
0/Lipids; 0/Membrane Lipids; 8072-95-5/suberin; EC 2.3.1.-/Acetyltransferases; EC 2.3.1.-/fatty acid elongases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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