Document Detail


Biochemical characterization of the cholesterol-dependent growth of the NS-1 mouse myeloma cell line.
MedLine Citation:
PMID:  3943557     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The biochemical basis for the cholesterol-dependent growth phenotype of the NS-1 myeloma cell line has been investigated. In one series of experiments, the growth response of NS-1 cells to several of the intermediates of cholesterol biosynthesis was studied in serum-free medium. The cholesterol precursors, squalene and lanosterol, were totally ineffective in promoting NS-1 cell growth. In contrast, cholesterol precursors downstream from lanosterol, i.e., desmosterol and 7-dehydrocholesterol, completely replaced cholesterol in supporting NS-1 cell growth. In a second series of experiments, NS-1 cells and NS-1-503 cells (a cholesterol growth-independent variant of NS-1 cells) were labelled with [2-14C]acetate and the distributions of radioactivity between cholesterol and its precursors were determined by thin-layer chromatography using two different solvent systems. The major labelled sterol product (greater than 80%) in NS-1 cells after a 24-h exposure to [2-14C]acetate was lanosterol. In contrast, the major labelled sterol product (greater than 95%) in NS-1-503 cells after a 24-h exposure to [2-14C]acetate was cholesterol. Taken together, these results indicate that NS-1 cells are defective in cholesterol biosynthesis and identify the site of lesion as the demethylation of lanosterol to C-29 sterol intermediates.
Authors:
J K Chen; T Okamoto; J D Sato; G H Sato; D B McClure
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Experimental cell research     Volume:  163     ISSN:  0014-4827     ISO Abbreviation:  Exp. Cell Res.     Publication Date:  1986 Mar 
Date Detail:
Created Date:  1986-03-24     Completed Date:  1986-03-24     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0373226     Medline TA:  Exp Cell Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  117-26     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Acetates / metabolism
Animals
Cell Division
Cell Line
Cholesterol / biosynthesis*,  pharmacology
Culture Media
Dehydrocholesterols / pharmacology
Desmosterol / pharmacology
Lanosterol / analogs & derivatives,  metabolism,  pharmacology
Mice
Multiple Myeloma / metabolism*,  pathology
Phenotype
Squalene / pharmacology
Sterols / metabolism
Chemical
Reg. No./Substance:
0/Acetates; 0/Culture Media; 0/Dehydrocholesterols; 0/Sterols; 111-02-4/Squalene; 128-33-6/zymosterol; 313-04-2/Desmosterol; 434-16-2/7-dehydrocholesterol; 57-88-5/Cholesterol; 79-62-9/lanostenol; 79-63-0/Lanosterol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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