Document Detail


Biochemical changes during the aerobic-anaerobic transition in Ascaris suum larvae.
MedLine Citation:
PMID:  3033496     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Ascaris suum L3 larvae isolated from rabbit lungs undergo the third ecdysis to L4 larvae after 3 days in culture under a gas phase of 85% N2/10% CO2/5% O2. The L3 larvae contain substantial malic enzyme activity and are capable of producing small amounts of the reduced organic acids characteristic of the fermentative pathways which operate in the adult. However, only a small portion of the total carbon utilized is accounted for by these reduced acids and their motility is cyanide-sensitive, suggesting that their energy-generating pathways are predominantly aerobic. In contrast, after ecdysis, the L4 larvae begin to utilize glucose at a greater rate and the proportion of total carbon utilized which is accounted for as propionate, 2-methylbutyrate and 2-methylvalerate also increases. In addition, motility becomes increasingly cyanide-insensitive, suggesting that these L4 larvae are able to utilize the anaerobic energy-generating pathways of the adult. Surprisingly, on day 10 in culture, these L4 larvae, although capable of producing reduced volatile acids, still retain substantial cyanide-sensitive cytochrome oxidase activity.
Authors:
P R Komuniecki; L Vanover
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Molecular and biochemical parasitology     Volume:  22     ISSN:  0166-6851     ISO Abbreviation:  Mol. Biochem. Parasitol.     Publication Date:  1987 Jan 
Date Detail:
Created Date:  1987-05-26     Completed Date:  1987-05-26     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  8006324     Medline TA:  Mol Biochem Parasitol     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  241-8     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Aerobiosis
Anaerobiosis
Animals
Ascaris / enzymology,  growth & development,  metabolism*
Butyrates / biosynthesis
Cyanides / pharmacology
Electron Transport Complex IV / metabolism
Energy Metabolism
Fatty Acids, Volatile / biosynthesis*
Glucose / metabolism
Larva / enzymology,  metabolism
Malate Dehydrogenase / metabolism
Movement / drug effects
Oxidation-Reduction
Pentanoic Acids / biosynthesis
Propionates / biosynthesis
Chemical
Reg. No./Substance:
0/Butyrates; 0/Cyanides; 0/Fatty Acids, Volatile; 0/Pentanoic Acids; 0/Propionates; 116-53-0/2-methylbutyrate; 50-99-7/Glucose; 97-61-0/2-methylvaleric acid; EC 1.1.1.37/Malate Dehydrogenase; EC 1.9.3.1/Electron Transport Complex IV

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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