| Biochemical and cell biological analyses of a mammalian septin complex, Sept7/9b/11. | |
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MedLine Citation:
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PMID: 15485874 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Septins are members of a conserved family of cytoskeletal GTPases present in organisms as diverse as yeast and mammals. Unlike lower eukaryotic cells, the physiological significance of mammalian septin complexes is largely unknown. Using specific antibodies, we found at least five septins, Sept2, Sept7, Sept8, Sept9b, and Sept11, in septin complexes affinity-purified with anti-Sept7 antibody-conjugated column from rat embryonic fibroblast REF52 cells. Immunofluorescence studies revealed co-localization of Sept7, Sept9b, and Sept11 along stress fibers in REF52 cells. Biochemical and immunoprecipitation analyses revealed that the three septins directly bind with each other through their N- or C-terminal divergent regions. These septins per se formed distinct and characteristic filament structures when transiently expressed in COS7 cells. When two of the three septins were co-expressed in COS7 cells, combination-dependent filament elongation, bundling, or disruption was observed. Taken together, our results suggest that septin filament structures may be affected by interactions with other septins included in the complex. |
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Authors:
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Koh-ichi Nagata; Tomiko Asano; Yoshinori Nozawa; Masaki Inagaki |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2004-10-12 |
Journal Detail:
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Title: The Journal of biological chemistry Volume: 279 ISSN: 0021-9258 ISO Abbreviation: J. Biol. Chem. Publication Date: 2004 Dec |
Date Detail:
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Created Date: 2004-12-23 Completed Date: 2005-02-25 Revised Date: 2009-11-23 |
Medline Journal Info:
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Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: United States |
Other Details:
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Languages: eng Pagination: 55895-904 Citation Subset: IM |
Affiliation:
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Department of Molecular Neurobiology, the Institute for Developmental Research, Aichi Human Service Center, 713-8 Kamiya-Cho, Kasugai, Aichi 480-0392, Japan. knagata@inst-hsc.jp |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blotting, Western COS Cells Cell Cycle Proteins / chemistry, physiology* Cell Line DNA, Complementary / metabolism Fibroblasts / metabolism GTP Phosphohydrolases / chemistry, physiology* Glutathione Transferase / metabolism Hela Cells Humans Immunoprecipitation Microscopy, Fluorescence Plasmids / metabolism Protein Binding Protein Structure, Tertiary Rats Recombinant Proteins / chemistry |
| Chemical | |
Reg. No./Substance:
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0/Cell Cycle Proteins; 0/DNA, Complementary; 0/Recombinant Proteins; 0/SEPT11 protein, human; EC 2.5.1.18/Glutathione Transferase; EC 3.6.1.-/GTP Phosphohydrolases; EC 3.6.1.-/SEPT7 protein, human; EC 3.6.1.-/SEPT9 protein, human |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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