| Biochemical and biological analysis of human interleukin 6 expressed in rodent and primate cells. | |
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MedLine Citation:
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PMID: 1715769 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The cDNA for human interleukin 6 (IL 6) was stably expressed at high levels in the three mammalian cell lines COS-7, PA317, and GH3 to yield IL 6 proteins of 25 to 27, 26, 22 to 24, and 23 kDa molecular mass. Both size and relative amounts of the recombinant IL 6 (rIL 6) species produced correspond to those of natural IL 6 secreted by LPS-stimulated monocytes. Oligosaccharide analysis of recombinant IL 6 utilizing tunicamycin and endoglycosidases revealed O- and N-linked glycosylation that is comparable to that of natural IL 6 derived from human monocytes and fibroblasts. IL 6 expressed in each of the three cell lines was phosphorylated similarly to the IL 6 produced in human monocytes and fibroblasts. IL 6 secreted by the three different cell lines have marked differences in specific biological activities. COS-7 IL 6 appeared to be 12-fold more active in its hybridoma growth factor activity than that made in PA317 or GH3 cells. In contrast, PA317 and GH3 IL 6 were 230 and 6.7 times more effective than COS-7 IL 6 in inducing Ig production in CESS cells. Also, PA317 and GH3 IL 6 were more effective than COS-7 IL 6 in inducing the acute-phase protein fibrinogen in human hepatocytes. The rIL 6 species exhibited no antiviral activity. |
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Authors:
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J E Tanner; N D Goldman; G Tosato |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Cytokine Volume: 2 ISSN: 1043-4666 ISO Abbreviation: Cytokine Publication Date: 1990 Sep |
Date Detail:
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Created Date: 1991-10-08 Completed Date: 1991-10-08 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9005353 Medline TA: Cytokine Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 363-74 Citation Subset: IM |
Affiliation:
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Division of Cytokine Biology, Food and Drug Administration, Bethesda, MD 20892. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acute-Phase Proteins
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biosynthesis Animals Cell Line Cercopithecus aethiops DNA Replication / drug effects Glycosylation Hemolytic Plaque Technique Humans Interleukin-6 / analysis, biosynthesis, genetics*, pharmacology Mice Plasmids Protein Biosynthesis Rats Recombinant Proteins / analysis, biosynthesis, pharmacology Transcription, Genetic Transfection* Tunicamycin / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Acute-Phase Proteins; 0/Interleukin-6; 0/Recombinant Proteins; 11089-65-9/Tunicamycin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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