| Biochemical basis for the killing of Cryptococcus neoformans by rat peritoneal cells. | |
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MedLine Citation:
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PMID: 7738723 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The biochemical basis of peritoneal cell cytotoxicity for Cryptococcus neoformans was studied by measuring the killing of the yeast by peritoneal resident cells and peritoneal exudate cells obtained from normal and proteose-peptone-injected animals, respectively. Both cell populations killed C. neoformans to an equivalent extent after 3 h incubation. Exudate cells showed anti-cryptococcal activity from the first hour of incubation, while no killing was observed with resident cells before 3 h. Both cell populations triggered a respiratory burst in response to opsonized C. neoformans as indicated by the fact that killing of the yeast was inhibited by scavengers of reactive oxygen intermediates (ROI). C. neoformans susceptibility to H2O2 and hydroxyl radicals in cell-free systems is demonstrated by incubating a yeast suspension with different concentrations of H2O2 and Fenton's reagents, respectively. These results suggest that oxygen metabolites play an active role in C. neoformans killing. |
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Authors:
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G R Rossi; D A Sastre; H R Rubinstein; D T Masih |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of medical and veterinary mycology : bi-monthly publication of the International Society for Human and Animal Mycology Volume: 32 ISSN: 0268-1218 ISO Abbreviation: J. Med. Vet. Mycol. Publication Date: 1994 Dec |
Date Detail:
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Created Date: 1995-06-08 Completed Date: 1995-06-08 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8605493 Medline TA: J Med Vet Mycol Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 405-14 Citation Subset: IM |
Affiliation:
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Departamento de Bioquímica Clínica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Argentina. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cells, Cultured Cryptococcus neoformans / physiology* Female Ferrous Compounds / pharmacology Hydrogen Peroxide / pharmacology Male Peritoneum / cytology* Rats Rats, Wistar Reactive Oxygen Species / metabolism Respiratory Burst / physiology |
| Chemical | |
Reg. No./Substance:
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0/Ferrous Compounds; 0/Reactive Oxygen Species; 7720-78-7/ferrous sulfate; 7722-84-1/Hydrogen Peroxide |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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