Document Detail


Biochemical analysis of transcriptional activation by Jun: differential activity of c- and v-Jun.
MedLine Citation:
PMID:  2510934     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The human proto-oncogene product, c-Jun, is a member of the AP-1 family of transcription factors, which mediate the regulation of gene expression in response to extracellular signaling. Comparison of c-Jun and v-Jun by in vitro transcription assays revealed that v-Jun has significantly greater transcriptional activity than c-Jun. Analysis of Jun mutants expressed in bacteria indicates that this difference in transcriptional activity is due to the presence of a regulatory domain located at the N-terminal region of c-Jun. Other Jun mutants identify an activation domain rich in acidic and proline residues toward the C-terminal end of the molecule, in a region near the DNA binding domain. These findings suggest that during retroviral transduction, a constitutively active Jun protein has been generated by deleting a negatively acting domain. This putative repressor domain may also play a role in the signal-dependent induction of c-Jun activity.
Authors:
D Bohmann; R Tjian
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Cell     Volume:  59     ISSN:  0092-8674     ISO Abbreviation:  Cell     Publication Date:  1989 Nov 
Date Detail:
Created Date:  1990-01-05     Completed Date:  1990-01-05     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0413066     Medline TA:  Cell     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  709-17     Citation Subset:  IM    
Affiliation:
Department of Biochemistry, University of California, Berkeley 94720.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Cell Transformation, Viral
Cloning, Molecular
DNA-Binding Proteins / genetics,  metabolism*
Escherichia coli / genetics
Gene Expression Regulation*
Hela Cells / metabolism
Humans
Molecular Sequence Data
Mutation
Oncogene Protein p65(gag-jun)
Plasmids
Protein Processing, Post-Translational
Protein-Tyrosine Kinases / metabolism
Proto-Oncogene Proteins / metabolism
Proto-Oncogene Proteins c-jun
Proto-Oncogenes*
Retroviridae Proteins, Oncogenic / metabolism*
Transcription Factors / genetics,  metabolism*
Transcription, Genetic*
Chemical
Reg. No./Substance:
0/DNA-Binding Proteins; 0/Oncogene Protein p65(gag-jun); 0/Proto-Oncogene Proteins; 0/Proto-Oncogene Proteins c-jun; 0/Retroviridae Proteins, Oncogenic; 0/Transcription Factors; EC 2.7.10.1/Protein-Tyrosine Kinases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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