Document Detail

Biochemical analysis of selenoprotein expression in brain cell lines and in distinct brain regions.
MedLine Citation:
PMID:  18317814     Owner:  NLM     Status:  MEDLINE    
Selenium is present in various biologically important selenoproteins. The preferential incorporation of selenium into the brain indicates its significance for this organ, but so far knowledge concerning the cerebral selenoproteome is scarce. We therefore investigated the expression of selenoproteins in various regions of the rat brain, various subcellular fractions and several brain cell lines by (75)Se-labelling, gel electrophoretic separation and autoradiography, with the (75)Se tracer as the selenoprotein marker. Quantitative evaluation of the labelled proteins in selenium-deficient rats revealed information regarding preferentially supplied selenoproteins and their distribution; 21 selenoproteins could be distinguished, among them a novel or modified 15-kDa selenoprotein enriched in the cerebellum cytosol. The selenoproteins differed in the degree of their expression among the brain regions and within a region among the subcellular fractions. Some cell-type-specific selenium-containing proteins were found in the cell lines. Differences in the distribution patterns between mono-cultured and co-cultured endothelial cells and astrocytes showed that mediators produced by other cells could affect the selenoprotein expression of a specific cell-type. This effect might play a role in the uptake and distribution of selenium in the brain but could also be of significance in the selenium metabolism of other tissues.
Barbara Hoppe; Anja U Bräuer; Markus Kühbacher; Nicolai E Savaskan; Dietrich Behne; Antonios Kyriakopoulos
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-03-04
Journal Detail:
Title:  Cell and tissue research     Volume:  332     ISSN:  0302-766X     ISO Abbreviation:  Cell Tissue Res.     Publication Date:  2008 Jun 
Date Detail:
Created Date:  2008-05-12     Completed Date:  2008-08-15     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0417625     Medline TA:  Cell Tissue Res     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  403-14     Citation Subset:  IM    
Department of Molecular Trace Element Research in the Life Sciences, Hahn-Meitner-Institut, Berlin, Germany.
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MeSH Terms
Brain / anatomy & histology,  metabolism*
Cell Line
Coculture Techniques
Electrophoresis, Polyacrylamide Gel
Rats, Wistar
Selenoproteins / metabolism*
Subcellular Fractions / metabolism
Reg. No./Substance:

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