Document Detail

Biochemical activities of extracts from Hypericum perforatum L. 3rd Communication: modulation of peroxidase activity as a simple method for standardization.
MedLine Citation:
PMID:  10083979     Owner:  NLM     Status:  MEDLINE    
Alcoholic extracts from the herb "St. John's wort" (Hypericum perforatum L.) are widely used to counteract depressive situations, where the question on the mainly active principle is still under discussion. Thus, standardization of the drug on the basis of dry matter has been chosen instead of the popular leading component, hypericin. Inhibition of myeloperoxidase-catalyzed dimerization of enkephalins by Hypericum extracts has recently been reported. This method is based on the separation and quantification of enkephalin dimers by HPLC. In order to simplify this assay myeloperoxidase could be substituted by the cheaper horseradish peroxidase and the enkephalins by the amino acid tyrosine without loss of significance. In this communication we represent a more rapid photometric method based on peroxidase-catalyzed indole acetic acid oxidation suitable for quick, simple and economic drug standardization.
H Schempp; A Denke; E Mann; W Schneider; E F Elstner
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Arzneimittel-Forschung     Volume:  49     ISSN:  0004-4172     ISO Abbreviation:  Arzneimittelforschung     Publication Date:  1999 Feb 
Date Detail:
Created Date:  1999-05-04     Completed Date:  1999-05-04     Revised Date:  2000-12-18    
Medline Journal Info:
Nlm Unique ID:  0372660     Medline TA:  Arzneimittelforschung     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  115-9     Citation Subset:  IM    
Lehrstuhl für Phytopathologie, Labor für angewandte Biochemie, Technische Universität München, Freising-Weihenstephan, Germany.
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MeSH Terms
Chromatography, High Pressure Liquid
Enzyme Inhibitors / pharmacology*,  standards
Horseradish Peroxidase / metabolism
Hydrogen Peroxide
Indoleacetic Acids / chemistry
Peroxidase / antagonists & inhibitors*
Plant Extracts / chemistry,  pharmacology,  standards
Theales / chemistry*,  growth & development
Tyrosine / chemistry
Reg. No./Substance:
0/Enzyme Inhibitors; 0/Fertilizers; 0/Indoleacetic Acids; 0/Oxidants; 0/Plant Extracts; 55520-40-6/Tyrosine; 7722-84-1/Hydrogen Peroxide; EC 1.11.1.-/Horseradish Peroxidase; EC

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