Document Detail


Biochemical and Genetic Analysis of NAD(P)H:Quinone Oxidoreductase 1 (NQO1).
MedLine Citation:
PMID:  23045144     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
NAD(P)H:quinone oxidoreductase 1 (NQO1, DT-diaphorase, E.C. 1.6.99.2) is an FAD containing obligate two-electron reductase that catalyzes the reduction of a broad range of substrates. This unit will describe methods for the detection of NQO1 protein in formalin-fixed, paraffin-embedded tissues by immunohistochemistry; detection of NQO1 protein in fresh tissues or cell lines by immunoblot analysis; measurement of NQO1 catalytic activity in fresh and frozen tissues and cell lines using spectrophotometric assays based upon the reduction of 2,6-dichlorophenol-indophenol (DCPIP) or coupled menadione-cytochrome reduction; and determination of the NQO1*2 polymorphism by PCR-RFLP.
Authors:
David Siegel; Jadwiga K Kepa; David Ross
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Current protocols in toxicology / editorial board, Mahin D. Maines (editor-in-chief) ... [et al.]     Volume:  Chapter 4     ISSN:  1934-9262     ISO Abbreviation:  Curr Protoc Toxicol     Publication Date:  2007 May 
Date Detail:
Created Date:  2012-10-09     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9816330     Medline TA:  Curr Protoc Toxicol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  Unit4.22     Citation Subset:  IM    
Affiliation:
University of Colorado at Denver and Health Sciences Center, Denver, Colorado.
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