| Biochemical characteristics of the novel haloalkane dehalogenase DatA, isolated from the plant pathogen Agrobacterium tumefaciens C58. | |
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MedLine Citation:
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PMID: 21193677 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We report the biochemical characterization of a novel haloalkane dehalogenase, DatA, isolated from the plant pathogen Agrobacterium tumefaciens C58. DatA possesses a peculiar pair of halide-stabilizing residues, Asn-Tyr, which have not been reported to play this role in other known haloalkane dehalogenases. DatA has a number of other unique characteristics, including substrate-dependent and cooperative kinetics, a dimeric structure, and excellent enantioselectivity toward racemic mixtures of chiral brominated alkanes and esters. |
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Authors:
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Khomaini Hasan; Andrea Fortova; Tana Koudelakova; Radka Chaloupkova; Mayuko Ishitsuka; Yuji Nagata; Jiri Damborsky; Zbynek Prokop |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-12-30 |
Journal Detail:
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Title: Applied and environmental microbiology Volume: 77 ISSN: 1098-5336 ISO Abbreviation: Appl. Environ. Microbiol. Publication Date: 2011 Mar |
Date Detail:
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Created Date: 2011-02-25 Completed Date: 2011-05-23 Revised Date: 2011-09-13 |
Medline Journal Info:
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Nlm Unique ID: 7605801 Medline TA: Appl Environ Microbiol Country: United States |
Other Details:
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Languages: eng Pagination: 1881-4 Citation Subset: IM |
Affiliation:
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oschmidt Laboratories, Department of Experimental Biology and Research Centre for Toxic Compounds in the Environment, Faculty of Science, Masaryk University, Kamenice 5/A13, 625 00 Brno, Czech Republic. |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/AB478945 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Alkanes
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metabolism DNA, Bacterial / chemistry, genetics Esters / metabolism Hydrolases / genetics, isolation & purification*, metabolism* Molecular Sequence Data Plants / microbiology Protein Multimerization Rhizobium radiobacter / enzymology*, genetics, metabolism Sequence Analysis, DNA Stereoisomerism Substrate Specificity |
| Chemical | |
Reg. No./Substance:
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0/Alkanes; 0/DNA, Bacterial; 0/Esters; EC 3.-/Hydrolases; EC 3.8.1.5/haloalkane dehalogenase |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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