| Biochemical analysis of a novel lipolytic enzyme YvdO from Bacillus subtilis 168. | |
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MedLine Citation:
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PMID: 20378985 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The predicted amino acid sequence of Bacillus subtilis yvdO exhibits similarity to that of the proteins belonging to the patatin family of lipolytic enzymes. In the present study, YvdO was overproduced in Escherichia coli and purified, and its enzymatic properties were determined. YvdO hydrolyzed p-nitrophenyl fatty acid esters. The enzyme was specific to middle-chain fatty acids, and its optimum pH was approximately 7.5. It maintained 86% of its initial activity after incubation for 30 min at 80 degrees C, and its secondary structure was retained at up to 80 degrees C. Free myristic acid was detected as the product of the reaction with YvdO and 1-myristoly-2-lyso-sn-glycero-3-phosphocholine, while YvdO did not hydrolyze 1,2-dimyristoly-sn-glycero-3-phosphocholine. These results suggest that YvdO is a novel thermostable lipolytic enzyme that has the ability to hydrolyze lysophospholipids. |
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Authors:
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Shiro Kato; Tohru Yoshimura; Hisashi Hemmi; Ryuichi Moriyama |
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Publication Detail:
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Type: Journal Article Date: 2010-04-07 |
Journal Detail:
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Title: Bioscience, biotechnology, and biochemistry Volume: 74 ISSN: 1347-6947 ISO Abbreviation: Biosci. Biotechnol. Biochem. Publication Date: 2010 |
Date Detail:
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Created Date: 2010-05-06 Completed Date: 2010-08-10 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9205717 Medline TA: Biosci Biotechnol Biochem Country: Japan |
Other Details:
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Languages: eng Pagination: 701-6 Citation Subset: IM |
Affiliation:
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Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya University, Aichi, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Bacillus subtilis / enzymology*, genetics, metabolism Escherichia coli / enzymology, genetics, metabolism Fatty Acids / genetics, metabolism |
| Chemical | |
Reg. No./Substance:
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0/Fatty Acids |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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