Document Detail


Biocatalytic synthesis of acrylates in supercritical fluids: tuning enzyme activity by changing pressure.
MedLine Citation:
PMID:  8464910     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Supercritical fluids are a unique class of nonaqueous media in which biocatalytic reactions can occur. The physical properties of supercritical fluids, which include gas-like diffusivities and liquid-like densities, can be predictably controlled with changing pressure. This paper describes how adjustment of pressure, with the subsequent predictable changes of the dielectric constant and Hildebrand solubility parameter for fluoroform, ethane, sulfur hexafluoride, and propane, can be used to manipulate the activity of lipase in the transesterification of methylmethacrylate with 2-ethyl-1-hexanol. Of particular interest is that the dielectric constant of supercritical fluoroform can be tuned from approximately 1 to 8, merely by increasing pressure from 850 to 4000 psi (from 5.9 to 28 MPa). The possibility now exists to predictably alter both the selectivity and the activity of a biocatalyst merely by changing pressure.
Authors:
S V Kamat; B Iwaskewycz; E J Beckman; A J Russell
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  90     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  1993 Apr 
Date Detail:
Created Date:  1993-05-04     Completed Date:  1993-05-04     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2940-4     Citation Subset:  IM    
Affiliation:
Department of Chemical Engineering, University of Pittsburgh, PA 15261.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Acrylates / chemical synthesis*
Candida / enzymology
Catalysis
Lipase / metabolism*
Models, Theoretical
Pressure
Solubility
Temperature
Chemical
Reg. No./Substance:
0/Acrylates; EC 3.1.1.3/Lipase
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Crystal structure of CATGGCCATG and its implications for A-tract bending models.
Next Document:  Expression of the major protein kinase C substrate, the acidic 80-kilodalton myristoylated alanine-r...