Document Detail

Biocatalytic conversion of wheat bran hydrolysate using an immobilized GH43 beta-xylosidase.
MedLine Citation:
PMID:  18674896     Owner:  NLM     Status:  MEDLINE    
To investigate the concept of a xylosidase-based process for the continuous production of xylose from arabinoxylan-containing feedstocks, a beta-xylosidase from Bacillus halodurans C-125 was immobilized and deployed in packed bed reactor (PBR). Among the several immobilization methods tested, glutaraldehyde-mediated immobilization on chitosan was the best both in terms of immobilization and activity yields (91% and 72.9%, respectively). In batch experiments the immobilized enzyme hydrolyzed wheat bran hydrolysates quite efficiently, consuming nearly all xylobiose and xylotriose after 6h. Its reusability showed only a 50% decrease of its activity after 92h. Using the chitosan-immobilized beta-xylosidase in a PBR, xylose productivity was 7.2g xylose l(-1)h(-1) and the conversion factor was 0.55 (derived from initial xylose in the substrate). The operational stability of the PBR was good, because only 25% of productivity was lost after the treatment of three batches of substrate over a 72-h period.
Issam Smaali; Caroline Rémond; Yousr Skhiri; Michael J O'Donohue
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-07-31
Journal Detail:
Title:  Bioresource technology     Volume:  100     ISSN:  1873-2976     ISO Abbreviation:  Bioresour. Technol.     Publication Date:  2009 Jan 
Date Detail:
Created Date:  2008-09-15     Completed Date:  2008-12-29     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9889523     Medline TA:  Bioresour Technol     Country:  England    
Other Details:
Languages:  eng     Pagination:  338-44     Citation Subset:  IM    
Fractionnement des Agroressources et Emballage, URCA, UMR INRA 614, Reims Cedex 2, France.
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MeSH Terms
Bacillus / enzymology*
Dietary Fiber*
Enzymes, Immobilized / chemistry
Plant Extracts / chemistry*
Triticum / chemistry*
Xylans / chemistry*
Xylose / chemical synthesis*
Xylosidases / chemistry*
Reg. No./Substance:
0/Enzymes, Immobilized; 0/Plant Extracts; 0/Xylans; 0/Xylose; 9040-27-1/arabinoxylan; EC 3.2.1.-/Xylosidases; EC,4-beta-D-xylosidase

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