Document Detail


Bioavailability of rumen bacterial selenium in mice using tissue uptake technique.
MedLine Citation:
PMID:  9403136     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A tissue uptake experiment was conducted to determine the bioavailability of rumen bacterial Selenium (Se) in mice. The donor animal was wether fed a diet containing 0.2 mg Se/kg dietary dry matter (DM). Ruminal fluid was collected 2 h postprandially. Bacterial-rich precipitate was obtained by differential centrifugation of the ruminal fluids. This was later freeze-dried and mixed in the diet to be used in feeding the mice experiment. Thirty growing female mice with a body wt (mean +/- SD) of 21.4 +/- 0.74 g were housed in plastic cages (5 mice/cage) and allotted equally to three dietary treatments. Diet 1 and Diet 2 were formulated based on AIN-76, except that no Se supplementation in the form of selenite was made in the former. In Diet 3, rumen bacterial matter was 20% of the diet, which gave an equivalent of 0.1 mg Se/kg dietary DM. The other two diets, Diet 1 and Diet 2, had an Se content of 0.025 and 0.1 mg/kg dietary DM, respectively. A 7-d feeding commenced after 7 d of acclimatization of the semipurified diet. Results showed that those mice fed an Se- (selenite) supplemented diet (Diet 2) had higher (P < 0.05) tissue Se concentrations than those mice fed the other two diets. No statistical differences were observed on various tissue Se concentrations between Diet 1 and Diet 3, although the latter diet had higher values. Kidney and liver had the highest Se concentrations compared to the other tissues. This study concludes that bacterial Se collected from the rumen of wether is not fully available for absorption in the intestine of the mice.
Authors:
A B Serra; S D Serra; K Shinchi; T Fujihara
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biological trace element research     Volume:  58     ISSN:  0163-4984     ISO Abbreviation:  Biol Trace Elem Res     Publication Date:  1997 Sep 
Date Detail:
Created Date:  1998-01-07     Completed Date:  1998-01-07     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  7911509     Medline TA:  Biol Trace Elem Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  255-61     Citation Subset:  IM    
Affiliation:
Faculty of Life and Environmental Science, Shimane University, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Bacteria / isolation & purification,  metabolism*
Biological Availability
Diet
Female
Mice
Rumen / microbiology*
Selenium / administration & dosage,  pharmacokinetics*
Tissue Distribution
Chemical
Reg. No./Substance:
7782-49-2/Selenium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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