Document Detail


Bioassay of thyrotropin receptor antibodies with Chinese hamster ovary cells transfected with recombinant human thyrotropin receptor: clinical utility in children and adolescents with Graves disease.
MedLine Citation:
PMID:  9580758     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: The objective of this study was to compare the clinical utility of a new bioassay for thyrotropin (TSH) receptor antibodies (Abs) with the conventional radioreceptor assay and with measurement of thyroid peroxidase Abs in the diagnosis of Graves disease in childhood. STUDY DESIGN: Serum samples obtained from 22 children and adolescents with Graves disease (19 hyperthyroid, 3 in remission), 13 children and adolescents with chronic lymphocytic thyroiditis, and 17 normal children in a control group were evaluated. RESULTS: TSH receptor Abs were detected by bioassay in 10 (91%) of 11 patients with active Graves disease but in 0 of 2 patients in remission, 0 of 13 normal members of the control group, and 0 of 11 patients with chronic lymphocytic thyroiditis including 1 with thyrotoxicosis. The sensitivity and specificity of TSH receptor Abs detected by radioreceptor assay studied in the same 11 patients and in an additional 11 patients was similar to bioassay. In contrast, thyroid peroxidase Abs were detected in only 12 (71%) of 17 patients with Graves disease but in 11 of 11 patients with chronic lymphocytic thyroiditis and in 0 of 17 members of the control group. CONCLUSION: Bioassay of TSH receptor Abs is both sensitive and specific for the diagnosis of active Graves disease in the young. When cost and simplicity are considered, however, bioassay offers no advantage over radioreceptor assay for initial diagnostic screening. Rather, bioassay for TSH receptor Abs may be useful in thyrotoxic patients who are negative initially in the radioreceptor assay or in treated patients whose clinical picture is discordant with results in the radioreceptor assay.
Authors:
D Botero; R S Brown
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of pediatrics     Volume:  132     ISSN:  0022-3476     ISO Abbreviation:  J. Pediatr.     Publication Date:  1998 Apr 
Date Detail:
Created Date:  1998-05-28     Completed Date:  1998-05-28     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  0375410     Medline TA:  J Pediatr     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  612-8     Citation Subset:  AIM; IM    
Affiliation:
Department of Pediatrics, University of Massachusetts Medical School, Worcester, USA.
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Animals
Autoantibodies / analysis*
Biological Assay
CHO Cells
Case-Control Studies
Child
Child, Preschool
Cricetinae
Female
Graves Disease / diagnosis*
Humans
Iodide Peroxidase / immunology
Male
Radioimmunoassay
Radioligand Assay
Receptors, Thyrotropin / immunology*
Sensitivity and Specificity
Thyroiditis, Autoimmune / diagnosis
Transfection
Chemical
Reg. No./Substance:
0/Autoantibodies; 0/Receptors, Thyrotropin; EC 1.11.1.8/Iodide Peroxidase
Comments/Corrections
Comment In:
J Pediatr. 1998 Apr;132(4):569-70   [PMID:  9580750 ]

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