Document Detail


Bioassay for siderophore utilization by Candida albicans.
MedLine Citation:
PMID:  1907814     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A convenient plate assay which is sensitive to medium pH has been developed to evaluate potential siderophores of Candida albicans. Adding a siderophore to a filter paper disk on chemically defined Lee's agar (final pH 7.2) seeded with the test strain reversed the growth inhibitory effects of the supplemented (25-100 micrograms/ml) iron chelator ethylenediaminedi(o-hydroxyphenylacetic acid), to provide a zone of growth stimulation. This bioassay has been used to demonstrate the structure-activity relationships of ferrichrome and several water-soluble hydroxamate peptide building blocks of this natural siderophore. Of all compounds so evaluated, ferrichrome exhibited the best activity.
Authors:
A A Minnick; L E Eizember; J A McKee; E K Dolence; M J Miller
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Analytical biochemistry     Volume:  194     ISSN:  0003-2697     ISO Abbreviation:  Anal. Biochem.     Publication Date:  1991 Apr 
Date Detail:
Created Date:  1991-09-11     Completed Date:  1991-09-11     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370535     Medline TA:  Anal Biochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  223-9     Citation Subset:  IM    
Affiliation:
Department of Chemistry and Biochemistry, University of Notre Dame, Indiana 46556.
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MeSH Terms
Descriptor/Qualifier:
Candida albicans / metabolism*
Cell Division / physiology
Edetic Acid / pharmacology
Ferrichrome / metabolism
Hydrogen-Ion Concentration
Iron / metabolism*
Metals / metabolism
Methods
Peptides / metabolism
Chemical
Reg. No./Substance:
0/Metals; 0/Peptides; 15630-64-5/Ferrichrome; 5657-17-0/EDDA; 60-00-4/Edetic Acid; 7439-89-6/Iron

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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