| Bioassay for siderophore utilization by Candida albicans. | |
| | |
MedLine Citation:
|
PMID: 1907814 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
A convenient plate assay which is sensitive to medium pH has been developed to evaluate potential siderophores of Candida albicans. Adding a siderophore to a filter paper disk on chemically defined Lee's agar (final pH 7.2) seeded with the test strain reversed the growth inhibitory effects of the supplemented (25-100 micrograms/ml) iron chelator ethylenediaminedi(o-hydroxyphenylacetic acid), to provide a zone of growth stimulation. This bioassay has been used to demonstrate the structure-activity relationships of ferrichrome and several water-soluble hydroxamate peptide building blocks of this natural siderophore. Of all compounds so evaluated, ferrichrome exhibited the best activity. |
| | |
Authors:
|
A A Minnick; L E Eizember; J A McKee; E K Dolence; M J Miller |
Related Documents
:
|
10901674 - Reducing degradation of azo dye by zero-valent iron in aqueous solution 1311154 - Daphnetin: a novel antimalarial agent with in vitro and in vivo activity. 2559504 - In vitro activity of nalidixic acid and its iron (iii) complex on entamoeba histolytica. 9089804 - The effect of dietary iron deficiency on the fatty acid composition of plasma and eryth... 15353404 - Cyclosporin a produces distal renal tubular acidosis by blocking peptidyl prolyl cis-tr... 18194844 - Solid-state interaction of stearic acid with povidone and its effect on dissolution sta... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: Analytical biochemistry Volume: 194 ISSN: 0003-2697 ISO Abbreviation: Anal. Biochem. Publication Date: 1991 Apr |
Date Detail:
|
Created Date: 1991-09-11 Completed Date: 1991-09-11 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 0370535 Medline TA: Anal Biochem Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 223-9 Citation Subset: IM |
Affiliation:
|
Department of Chemistry and Biochemistry, University of Notre Dame, Indiana 46556. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Candida albicans
/
metabolism* Cell Division / physiology Edetic Acid / pharmacology Ferrichrome / metabolism Hydrogen-Ion Concentration Iron / metabolism* Metals / metabolism Methods Peptides / metabolism |
| Chemical | |
Reg. No./Substance:
|
0/Metals; 0/Peptides; 15630-64-5/Ferrichrome; 5657-17-0/EDDA; 60-00-4/Edetic Acid; 7439-89-6/Iron |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: A solid-phase assay for beta-1,4-galactosyltransferase activity in human serum using recombinant aeq...
Next Document: A method for preparing skeletal muscle fiber basal laminae.