Document Detail


Bioassay for nisin in sausage; a shelf life study of nisin in cooked sausage.
MedLine Citation:
PMID:  22060859     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
A bioassay for nisin, an antimicrobial peptide used as a food additive, was developed for sausages based on nisin induced GFP-fluorescence (green fluorescent protein), and that could be performed on a microplate. The amount of GFP-fluorescence was easily measurable and dependent on nisin concentration. This GFP-bioassay for nisin had a detection limit in sausage as low as 0.9 ppm (0.9 μg/g), and it was used to assay shelf life of nisin in cooked sausage. Of the added nisin 68% could be detected from the sausages after storage of 28 days at 6 °C.
Authors:
J Reunanen; P E J Saris
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Meat science     Volume:  66     ISSN:  0309-1740     ISO Abbreviation:  Meat Sci.     Publication Date:  2004 Mar 
Date Detail:
Created Date:  2011-11-08     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101160862     Medline TA:  Meat Sci     Country:  England    
Other Details:
Languages:  eng     Pagination:  515-8     Citation Subset:  -    
Affiliation:
Applied Chemistry and Microbiology, PO Box 56, FIN-00014 University of Helsinki, Finland.
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