| Bioassay-guided purification and identification of PPARalpha/gamma agonists from Chlorella sorokiniana. | |
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MedLine Citation:
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PMID: 18398904 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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This study isolated agonists of peroxisome proliferator activated receptors (PPARs) from the green algae Chlorella sorokiniana, using a bioassay-guided purification strategy. PPARs are widely recognized as the molecular drug targets for many diseases including hyperglycemia, diabetes, obesity and cancer. Two independent bioassays were developed. The first is the scintillation proximity assay, a ligand binding assay. The other is the cell-based transcriptional activation assay which uses the Dual-Luciferase reporter system as the reporter gene under the control of the PPAR response element. Using these two assays, a PPARgamma-active fraction, CE 3-3, was obtained from C. sorokiniana extracts, which was also able to activate PPARalphamediated gene expression. To elucidate the active ingredients in the CE 3-3 fraction, GC-MS analysis was employed. The results showed that the CE 3-3 fraction consisted of at least ten fatty acids (FAs). The bioactivities of several of the individual FAs were evaluated for their PPARgamma activity and the results showed that linolenic acid and linoleic acid were the most potent FAs tested. Our studies indicate that Chlorella sorokiniana could have potential health benefits through the dual activation of PPARalpha/gamma via its unique FA constituents. |
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Authors:
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Yu-Cheng Chou; Ekambaranellore Prakash; Chien-Fu Huang; Tzu-Wen Lien; Xin Chen; Ih-Jen Su; Yu-Sheng Chao; Hsing-Pang Hsieh; John Tsu-An Hsu |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Phytotherapy research : PTR Volume: 22 ISSN: 1099-1573 ISO Abbreviation: Phytother Res Publication Date: 2008 May |
Date Detail:
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Created Date: 2008-05-05 Completed Date: 2008-07-30 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 8904486 Medline TA: Phytother Res Country: England |
Other Details:
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Languages: eng Pagination: 605-13 Citation Subset: IM |
Affiliation:
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Division of Biotechnology and Pharmaceutical Research, National Health Research Institutes, Taiwan, ROC. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Biological Assay
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methods* Cell Line, Tumor Chlorella / chemistry* Fatty Acids / isolation & purification, pharmacology Gas Chromatography-Mass Spectrometry Humans Linoleic Acid / isolation & purification, pharmacology PPAR alpha / agonists*, genetics PPAR gamma / agonists*, genetics Transcriptional Activation / drug effects alpha-Linolenic Acid / isolation & purification, pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Fatty Acids; 0/PPAR alpha; 0/PPAR gamma; 2197-37-7/Linoleic Acid; 463-40-1/alpha-Linolenic Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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