| Bioactive responses of Hep-G2 cells to soyasaponin extracts differs with respect to extraction conditions. | |
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MedLine Citation:
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PMID: 19520140 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Soyasaponins are bioactive oleanane triterpenoids found in soy and other legumes. The effect of two methanolic extractions of soy flour, room temperature (RT) and reflux (RE) extractions on composition and bioactive properties in hepatocarcinoma cells (Hep-G2) were investigated. A greater amount of 2,3-dihydro-2,5-dihydroxy-6-methyl-4H-pyran-4-one (DDMP) conjugated soyasaponins betag was measured in RT and a greater amount of the structurally related non-DDMP soyasaponins I and III were detected in RE. MTT cell viability yielded an LC50 of 0.926+/-0.08 mg/mL for RT and 0.546+/-0.06 mg/mL for RE. ViaCount viability assay showed similar results for RE as the MTT assay however, RT treatment produced no difference compared with the control. Analysis using TUNEL and cell cycle analysis revealed that RE treatment induced apoptosis and flow cytometry forward side scatter and morphological assessment of RT showed evidence of Hep-G2 differentiation after 72 h. Differences in the bioactivities may be attributed to the different concentration of DDMP conjugated soyasaponin betag recovered in RT and RE extracts. |
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Authors:
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Wei Zhang; Mei Ching Yeo; Fang Yin Tang; David G Popovich |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-06-09 |
Journal Detail:
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Title: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association Volume: 47 ISSN: 1873-6351 ISO Abbreviation: Food Chem. Toxicol. Publication Date: 2009 Sep |
Date Detail:
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Created Date: 2009-08-11 Completed Date: 2009-10-13 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8207483 Medline TA: Food Chem Toxicol Country: England |
Other Details:
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Languages: eng Pagination: 2202-8 Citation Subset: IM |
Affiliation:
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Department of Chemistry, National University of Singapore, Singapore. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Antineoplastic Agents
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analysis,
pharmacology* Carcinoma, Hepatocellular / drug therapy*, pathology Cell Cycle / drug effects Cell Line, Tumor Cell Survival / drug effects Chromatography, High Pressure Liquid Drug Screening Assays, Antitumor Hepatocytes / drug effects*, metabolism, pathology Humans Liver Neoplasms / drug therapy*, pathology Oleanolic Acid / analogs & derivatives, analysis Plant Extracts / chemistry, pharmacology Saponins / analysis, chemistry, pharmacology* Soybean Proteins / analysis Spectrometry, Mass, Electrospray Ionization Tetrazolium Salts / metabolism Thiazoles / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Antineoplastic Agents; 0/Plant Extracts; 0/Saponins; 0/Soybean Proteins; 0/Tetrazolium Salts; 0/Thiazoles; 0/soyasaponin beta g; 298-93-1/thiazolyl blue; 508-02-1/Oleanolic Acid; 51330-27-9/soyasaponin I; 55319-36-3/soyasaponin II |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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