Document Detail


Bioactive responses of Hep-G2 cells to soyasaponin extracts differs with respect to extraction conditions.
MedLine Citation:
PMID:  19520140     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Soyasaponins are bioactive oleanane triterpenoids found in soy and other legumes. The effect of two methanolic extractions of soy flour, room temperature (RT) and reflux (RE) extractions on composition and bioactive properties in hepatocarcinoma cells (Hep-G2) were investigated. A greater amount of 2,3-dihydro-2,5-dihydroxy-6-methyl-4H-pyran-4-one (DDMP) conjugated soyasaponins betag was measured in RT and a greater amount of the structurally related non-DDMP soyasaponins I and III were detected in RE. MTT cell viability yielded an LC50 of 0.926+/-0.08 mg/mL for RT and 0.546+/-0.06 mg/mL for RE. ViaCount viability assay showed similar results for RE as the MTT assay however, RT treatment produced no difference compared with the control. Analysis using TUNEL and cell cycle analysis revealed that RE treatment induced apoptosis and flow cytometry forward side scatter and morphological assessment of RT showed evidence of Hep-G2 differentiation after 72 h. Differences in the bioactivities may be attributed to the different concentration of DDMP conjugated soyasaponin betag recovered in RT and RE extracts.
Authors:
Wei Zhang; Mei Ching Yeo; Fang Yin Tang; David G Popovich
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-06-09
Journal Detail:
Title:  Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association     Volume:  47     ISSN:  1873-6351     ISO Abbreviation:  Food Chem. Toxicol.     Publication Date:  2009 Sep 
Date Detail:
Created Date:  2009-08-11     Completed Date:  2009-10-13     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8207483     Medline TA:  Food Chem Toxicol     Country:  England    
Other Details:
Languages:  eng     Pagination:  2202-8     Citation Subset:  IM    
Affiliation:
Department of Chemistry, National University of Singapore, Singapore.
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MeSH Terms
Descriptor/Qualifier:
Antineoplastic Agents / analysis,  pharmacology*
Carcinoma, Hepatocellular / drug therapy*,  pathology
Cell Cycle / drug effects
Cell Line, Tumor
Cell Survival / drug effects
Chromatography, High Pressure Liquid
Drug Screening Assays, Antitumor
Hepatocytes / drug effects*,  metabolism,  pathology
Humans
Liver Neoplasms / drug therapy*,  pathology
Oleanolic Acid / analogs & derivatives,  analysis
Plant Extracts / chemistry,  pharmacology
Saponins / analysis,  chemistry,  pharmacology*
Soybean Proteins / analysis
Spectrometry, Mass, Electrospray Ionization
Tetrazolium Salts / metabolism
Thiazoles / metabolism
Chemical
Reg. No./Substance:
0/Antineoplastic Agents; 0/Plant Extracts; 0/Saponins; 0/Soybean Proteins; 0/Tetrazolium Salts; 0/Thiazoles; 0/soyasaponin beta g; 298-93-1/thiazolyl blue; 508-02-1/Oleanolic Acid; 51330-27-9/soyasaponin I; 55319-36-3/soyasaponin II

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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