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Bioactive Coronary Stent Coating Based on Layer-by-Layer Technology for siRNA Release.
MedLine Citation:
PMID:  23333865     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
OBJECTIVES: One procedure to treat stenotic coronary arteries is the percutaneous transluminal coronary angioplasty (PTCA). In recent years, drug eluting stents (DES) have demonstrated elaborate ways to improve outcomes of intravascular interventions. To enhance DESs, the idea has evolved to design stents that elute specific small interfering RNA (siRNA) for better vascular wall regeneration. The layer-by-layer (LbL) technology offers the possibility to incorporate siRNA nanoplexes (NPs) to achieve bioactive medical implant coatings. METHODS AND RESULTS: The LbL technique was used to achieve hyaluronic-acid/chitosan (HA/Chi) films with incorporated Chi-siRNA NPs. The multilayer growth was monitored by quartz crystal microbalance (QCM). The coating on stents and the thickness were analyzed using fluorescence and scanning electron microscopy (SEM). All stents showed homogenous coating, and the polyelectrolyte multilayers (PEMs) were not disrupted after ethylene oxide (ETO) sterilization or expansion. The in vitro uptake of fluorescent-labelled NPs from PEMs in primary human endothelial cells (ECs) was analyzed by flow cytometry for 2, 6, and 9 days. Furthermore, stents coated with HA/Chi and Chi-siRNA NPs were expanded into porcine arteries and showed ex vivo delivery of NPs. The films showed no critical results in terms of hemocompatibility. CONCLUSION: This study demonstrates that Chi-siRNA NPs can be incorporated into PEMs consisting of HA and Chi. We conclude that the NPs were delivered to ECs under in vitro conditions. Furthermore, under ex vivo conditions, NPs were transferred into porcine artery walls. Due to their good hemocompatibility, they might make an innovative tool for achieving bioactive coatings for coronary stents.
Authors:
S Hossfeld; A Nolte; H Hartmann; M Recke; M Schaller; T Walker; J Kjems; B Schlosshauer; D Stoll; H-P Wendel; R Krastev
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-17
Journal Detail:
Title:  Acta biomaterialia     Volume:  -     ISSN:  1878-7568     ISO Abbreviation:  Acta Biomater     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-21     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101233144     Medline TA:  Acta Biomater     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2013. Published by Elsevier Ltd.
Affiliation:
NMI - Natural and Medical Sciences Institute at the University of Tuebingen, Reutlingen, Germany.
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