Document Detail


Bio-smart hydrogels: co-joined molecular recognition and signal transduction in biosensor fabrication and drug delivery.
MedLine Citation:
PMID:  12392946     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Two classes of polymers that are currently receiving widespread attention in biosensor development are hydrogels and conducting electroactive polymers. The present study reports on the integration of these two materials to produce electroactive hydrogel composites that physically entrap enzymes within their matrices for biosensor construction and chemically stimulated controlled release. Enhanced biosensing capabilities of these membranes have been demonstrated in the fabrication of glucose, cholesterol and galactose amperometric biosensors. All biosensors displayed extended linear response ranges (10(-5)-10(-2) M), rapid response times (<60 s), retained storage stabilities of up to 1 year, and excellent screening of the physiological interferents ascorbic acid, uric acid, and acetaminophen. When the cross-linked hydrogel components of these composite membranes were prepared with the amine containing dimethylaminoethyl methacrylate monomer the result was polymeric devices that swelled in response to pH changes (neutral to acidic). Entrapment of glucose oxidase within these materials made them glucose-responsive through the formation of gluconic acid. When insulin was co-loaded with glucose oxidase into these "bio-smart" devices, there was a twofold increase in insulin release rate when the devices were immersed in glucose solutions. This demonstrates the potential of such systems to function as a chemically-synthesized artificial pancreas.
Authors:
Sean Brahim; Dyer Narinesingh; Anthony Guiseppi-Elie
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Publication Detail:
Type:  Evaluation Studies; Journal Article    
Journal Detail:
Title:  Biosensors & bioelectronics     Volume:  17     ISSN:  0956-5663     ISO Abbreviation:  Biosens Bioelectron     Publication Date:  2002 Dec 
Date Detail:
Created Date:  2002-10-23     Completed Date:  2003-06-18     Revised Date:  2009-07-14    
Medline Journal Info:
Nlm Unique ID:  9001289     Medline TA:  Biosens Bioelectron     Country:  England    
Other Details:
Languages:  eng     Pagination:  973-81     Citation Subset:  IM    
Affiliation:
Department of Chemical Engineering and Center for Bioelectronics, Biosensors and Biochips (C3B), Virginia Commonwealth University, P.O. Box 843038, 601 West Main Street, Richmond, VA 23284-3038, USA.
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MeSH Terms
Descriptor/Qualifier:
Biocompatible Materials*
Biosensing Techniques / instrumentation*,  methods
Cholesterol / analysis,  chemistry
Electrochemistry / instrumentation,  methods
Enzymes, Immobilized*
Equipment Design
Galactose / analysis,  chemistry
Glucose / analysis,  chemistry
Hydrogels*
Hydrogen-Ion Concentration
Infusion Pumps, Implantable
Insulin Infusion Systems*
Membranes, Artificial
Methacrylates
Oxidoreductases / chemistry*
Polymers*
Pyrroles
Quality Control
Sensitivity and Specificity
Chemical
Reg. No./Substance:
0/Biocompatible Materials; 0/Enzymes, Immobilized; 0/Hydrogels; 0/Membranes, Artificial; 0/Methacrylates; 0/Polymers; 0/Pyrroles; 26566-61-0/Galactose; 2867-47-2/2-(dimethylamino)ethyl methacrylate; 30604-81-0/polypyrrole; 50-99-7/Glucose; 57-88-5/Cholesterol; 868-77-9/hydroxyethyl methacrylate; EC 1.-/Oxidoreductases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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