Document Detail

Bio-functionalized thermoresponsive interfaces facilitating cell adhesion and proliferation.
MedLine Citation:
PMID:  16782188     Owner:  NLM     Status:  MEDLINE    
Bio-functionalized thermoresponsive culture interfaces co-immobilized with cell adhesive peptide, RGDS, and cell growth factor, insulin (INS), are investigated to promote initial cell adhesion and cell growth for further cell sheet engineering applications. These bio-functionalized interfaces were prepared by electron beam-induced copolymerization of N-isopropylacrylamide (IPAAm) with its carboxyl-derivatized analog, 2-carboxyisopropylacrylamide (CIPAAm), and grafting onto tissue culture polystyrene dishes, followed by immobilization of RGDS and/or INS to CIPAAm carboxyls. Adhesion and proliferation of bovine carotid artery endothelial cells (ECs) were examined on the RGDS-INS co-immobilized thermoresponsive interfaces. Immobilized RGDS facilitated initial EC adhesion on the surfaces and INS modification was demonstrated to induce EC proliferation, respectively. More pronounced EC growth was indicated by co-immobilization of appropriate amount of RGDS and INS. This may be due to synergistic effect of direct co-stimulation of adhered ECs by surface-immobilized RGDS and INS molecules. ECs grown on the RGDS-INS co-immobilized thermoresponsive interfaces can also be recovered spontaneously as viable tissue monolayers by solely reducing culture temperature. RGDS-INS co-immobilized thermoresponsive interfaces strongly supported initial EC adhesion and growth than unmodified thermoresponsive surfaces even under serum-free culture. Addition of soluble growth factors to serum-free culture medium effectively induced EC proliferation to confluency. Co-immobilization of cell adhesion peptides and growth factors on thermoresponsive surfaces should be effective for rapid preparation of intact cell sheets and their utilization to regenerative medicine.
Hideyuki Hatakeyama; Akihiko Kikuchi; Masayuki Yamato; Teruo Okano
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-06-19
Journal Detail:
Title:  Biomaterials     Volume:  27     ISSN:  0142-9612     ISO Abbreviation:  Biomaterials     Publication Date:  2006 Oct 
Date Detail:
Created Date:  2006-07-03     Completed Date:  2006-12-22     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8100316     Medline TA:  Biomaterials     Country:  England    
Other Details:
Languages:  eng     Pagination:  5069-78     Citation Subset:  IM    
Institute of Advanced Biomedical Engineering and Science, Center of Excellence (COE) Program for the 21st Century, Tokyo Women's Medical University, Tokyo, Japan.
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MeSH Terms
Biocompatible Materials*
Cell Adhesion / physiology
Cell Proliferation*
Cells, Cultured
Endothelial Cells / physiology
Reg. No./Substance:
0/2-carboxyisopropylacrylamide; 0/Acrylamides; 0/Biocompatible Materials; 0/Oligopeptides; 0/Polystyrenes; 11061-68-0/Insulin; 2210-25-5/N-isopropylacrylamide; 91037-65-9/arginyl-glycyl-aspartyl-serine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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