Document Detail

Bio-electrosprays: the next generation of electrified jets.
MedLine Citation:
PMID:  16941448     Owner:  NLM     Status:  MEDLINE    
Biological electrosprays are rapidly becoming a robust means by which to engineer living organisms for applications ranging from tissue repair to developmental biology. We previously reported the ability to electrospray living organisms without compromising their viability, but found it challenging to achieve stability in the jetting of these organisms as a result of the chemical properties of the living cellular suspensions. Jet stability is required for the generation of a near-mono distribution of droplets, which is necessary for the development of electrospray technology as a "drop and place" biotechnique. Recently, we determined the conditions needed to achieve jet stability and were able to generate droplets with a near-mono distribution (<50 microm). In this communication, we elucidate the relationship between jet behaviour and droplet size under stable jetting conditions, with a view to further reducing the droplet size to deposit a single living cell within a droplet. We believe that this level of resolution will make electrospray jetting superior amongst the jet-based biotechnologies presently being developed for the engineering of biological architectures comprised of living cells.
Suwan N Jayasinghe; Andrea Townsend-Nicholson
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biotechnology journal     Volume:  1     ISSN:  1860-7314     ISO Abbreviation:  Biotechnol J     Publication Date:  2006 Sep 
Date Detail:
Created Date:  2006-09-25     Completed Date:  2007-01-03     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  101265833     Medline TA:  Biotechnol J     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  1018-22     Citation Subset:  IM    
Department of Mechanical Engineering, University College London, London, UK.
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MeSH Terms
Biotechnology / instrumentation,  methods*
Cell Culture Techniques / instrumentation
Cell Line
Cell Survival
Electrochemistry / methods
Nanotechnology / instrumentation,  methods*
Particle Size
Physical Phenomena

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