Document Detail


Bio-electrospraying primary cardiac cells: in vitro tissue creation and functional study.
MedLine Citation:
PMID:  21053334     Owner:  NLM     Status:  In-Process    
Abstract/OtherAbstract:
Manifestations of myocardial infarctions have been recognized as one of the major killers in the Western world. Therefore, advancing and developing novel cardiac tissue repair and replacement therapeutics have great implications to our health sciences and well-being. There are several approaches for forming cardiac tissues, non-jet-based and jet-based methodologies. A unique advantage of jet-based approaches is the possibility to handle living cells with a matrix for cell distribution and deposition in suspension, either as single or heterogeneous cell populations. Our previous studies on bio-electrospraying of cardiac cells have shown great promise. Here, we show for the first time the ability to bio-electrospray the three major cell types of the myocardium, both independently and simultaneously, for forming a fully functional cardiac tissue. Several samples are characterized in vitro and found to be indistinguishable in comparison to controls. Thus, we are describing a swiftly emerging novel biotechnique for direct cardiac tissue generation. Moreover, the present investigations pave the way for the development and optimization of a bio-patterning approach for the fabrication of biologically viable cardiac tissue grafts for the potential treatment of severe heart failure after myocardial infarction.
Authors:
Keat-Eng Ng; Pascal Joly; Suwan N Jayasinghe; Bertrand Vernay; Richard Knight; Sean P Barry; James McComick; David Latchman; Anastasis Stephanou
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biotechnology journal     Volume:  6     ISSN:  1860-7314     ISO Abbreviation:  Biotechnol J     Publication Date:  2011 Jan 
Date Detail:
Created Date:  2011-01-12     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101265833     Medline TA:  Biotechnol J     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  86-95     Citation Subset:  IM    
Affiliation:
Medical Molecular Biology Unit, University College London, Institute of Child Health, 30 Guilford Street, London, WC1N 1EH, UK.
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MeSH Terms
Descriptor/Qualifier:
Grant Support
ID/Acronym/Agency:
//British Heart Foundation

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