| Binding to the yeast SwI4,6-dependent cell cycle box, CACGAAA, is cell cycle regulated in vivo. | |
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MedLine Citation:
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PMID: 8604294 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In Saccharomyces cerevisiae commitment to cell division occurs late in the G1 phase of the cell cycle at a point called Start and requires the activity of the Cdc28 protein kinase and its associated G1 cyclins. The Swi4,6-dependent cell cycle box binding factor, SBF, is important for maximal expression of the G1 cyclin and HO endonuclease genes at Start. The cell cycle regulation of these genes is modulated through an upstream regulatory element termed the SCB (SwI4,6-dependent cell cycle box, CACGAAA), which is dependent on both SWI4 and SWI6. Although binding of SWI4 and SWI6 to SCB sequences has been well characterized in vitro, the binding of SBF in vivo has not been examined. We used in vivo dimethyl sulfate footprinting to examine the occupancy of SCB sequences throughout the cell cycle. We found that binding to SCB sequences occurred in the G1 phase of the cell cycle and was greatly reduced in G2. In the absence of either SWI4 or SWI6, SCB sequences were not occupied at any cell cycle stage. These results suggest that the G1-specific expression of SCB-dependent genes is regulated at the level of DNA binding in vivo. |
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Authors:
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L A Harrington; B J Andrews |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Nucleic acids research Volume: 24 ISSN: 0305-1048 ISO Abbreviation: Nucleic Acids Res. Publication Date: 1996 Feb |
Date Detail:
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Created Date: 1996-05-15 Completed Date: 1996-05-15 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 0411011 Medline TA: Nucleic Acids Res Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 558-65 Citation Subset: IM |
Affiliation:
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Department of Molecular and Medical Genetics, University of Toronto, Ontario, Canada. |
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| MeSH Terms | |
Descriptor/Qualifier:
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Base Sequence Binding Sites Cell Cycle / genetics DNA-Binding Proteins Fungal Proteins / genetics Genes, Fungal* Molecular Sequence Data Saccharomyces cerevisiae / cytology*, genetics Saccharomyces cerevisiae Proteins* Transcription Factors / genetics Transcription, Genetic |
| Chemical | |
Reg. No./Substance:
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0/DNA-Binding Proteins; 0/Fungal Proteins; 0/SWI4 protein, S cerevisiae; 0/SWI6 protein, S cerevisiae; 0/Saccharomyces cerevisiae Proteins; 0/Transcription Factors |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
| Full Text | |
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Journal Information Journal ID (nlm-ta): Nucleic Acids Res ISSN: 0305-1048 ISSN: 1362-4962 |
Article Information Download PDF  Print publication date: Day: 15 Month: 2 Year: 1996 Volume: 24 Issue: 4 First Page: 558 Last Page: 565 ID: 145676 PubMed Id: 8604294 Publisher Item Identifier: 5e0717 |
| Binding to the yeast SwI4,6-dependent cell cycle box, CACGAAA, is cell cycle regulated in vivo. | |
| L A Harrington | |
| B J Andrews | |
| Department of Molecular and Medical Genetics, University of Toronto, Ontario, Canada. |
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