| Binding sites of muscarinic and adrenergic receptors in gastrointestinal tissues of dairy cows suffering from left displacement of the abomasum. | |
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MedLine Citation:
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PMID: 19796972 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Muscarinic acetylcholine (M) and adrenergic (AR) receptors mediate gastrointestinal motility. Using radioligand binding assays and real-time polymerase chain reaction, the densities of binding sites and mRNA levels of M₂, M₃, α₂(AD)- and β₂-AR were compared in muscle tissues from the abomasal fundus, pylorus, duodenum, caecum, and external loop of the spiral colon of eight cows with left displacement of abomasum (LDA), and of eight healthy cows. Specific binding of the [³H]-ligands to each of the four receptors was competitive and saturable. Binding sites of M₂ (all intestinal sites), M₃ (duodenum and caecum), and of α₂(AD)-AR (abomasal fundus) were lower (P < 0.05) in cows with LDA than in healthy cows. The coefficients of correlation between binding sites and mRNA transcripts of receptors were dissimilar in cows with LDA and healthy cows. The decrease in densities of M (intestine) and of α₂(AD)-AR (abomasum) receptors suggests their implication in the impairment of motility associated with or leading to LDA. |
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Authors:
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E C Ontsouka; M Niederberger; A Steiner; R M Bruckmaier; M Meylan |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-09-30 |
Journal Detail:
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Title: Veterinary journal (London, England : 1997) Volume: 186 ISSN: 1532-2971 ISO Abbreviation: Vet. J. Publication Date: 2010 Dec |
Date Detail:
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Created Date: 2010-12-06 Completed Date: 2011-01-06 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9706281 Medline TA: Vet J Country: England |
Other Details:
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Languages: eng Pagination: 328-37 Citation Subset: IM |
Copyright Information:
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Copyright © 2009 Elsevier Ltd. All rights reserved. |
Affiliation:
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Clinic for Ruminants, Vetsuisse Faculty, University of Berne, CH-3012 Berne, Switzerland. edgar.ontsouka@physio.unibe.ch |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Abomasum* Animals Binding Sites Cattle Cattle Diseases / metabolism* Female Gastrointestinal Motility / physiology* RNA, Messenger / genetics, metabolism Receptors, Adrenergic / metabolism* Receptors, Muscarinic / metabolism* Stomach Diseases / metabolism, veterinary* |
| Chemical | |
Reg. No./Substance:
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0/RNA, Messenger; 0/Receptors, Adrenergic; 0/Receptors, Muscarinic |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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