Document Detail


Binding of serum mannan binding lectin to a cell integrity-defective Cryptococcus neoformans ccr4Delta mutant.
MedLine Citation:
PMID:  17646356     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Mannan binding lectin (MBL) is an innate immune mediator belonging to the collectin family known to bind to the surfaces of many viruses, bacteria, and fungi. However, pathogenic strains of the fungus Cryptococcus neoformans are resistant to MBL binding. To dissect the mechanism of cryptococcal resistance to MBL, we compared MBL binding to an encapsulated wild-type strain, an encapsulated ccr4Delta mutant defective in cell integrity, and an acapsular cap60Delta strain. No MBL binding was detected on wild-type C. neoformans. In contrast, the ccr4Delta mutant bound MBL to the cell wall, predominantly at the ends of enlarged buds, whereas the acapsular strain bound MBL only at the bud neck and bud scars. In addition, the ccr4Delta mutant was sensitive to the cell wall-active antifungal caspofungin and other cell wall stress inducers, and its virulence was reduced in a mouse model of cryptococcosis. Interestingly, treatment of wild-type cells with caspofungin also increased MBL binding to C. neoformans. These results suggest that both the presence of capsule and wild-type cell wall architecture preclude MBL binding to C. neoformans.
Authors:
John C Panepinto; Kazimierz W Komperda; Moshe Hacham; Soowan Shin; Xiaoguang Liu; Peter R Williamson
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2007-07-23
Journal Detail:
Title:  Infection and immunity     Volume:  75     ISSN:  0019-9567     ISO Abbreviation:  Infect. Immun.     Publication Date:  2007 Oct 
Date Detail:
Created Date:  2007-09-20     Completed Date:  2007-10-31     Revised Date:  2013-06-06    
Medline Journal Info:
Nlm Unique ID:  0246127     Medline TA:  Infect Immun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  4769-79     Citation Subset:  IM    
Affiliation:
University of Illinois at Chicago, Section of Infectious Diseases, Chicago, IL 60612, USA. panepijc@uic.edu
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MeSH Terms
Descriptor/Qualifier:
Animals
Antifungal Agents / pharmacology
Cell Wall / chemistry
Cryptococcosis / immunology*,  microbiology
Cryptococcus neoformans / genetics,  immunology,  metabolism*,  pathogenicity*
Echinocandins
Gene Deletion
Mannose-Binding Lectin / immunology,  metabolism*
Mice
Microbial Sensitivity Tests
Peptides, Cyclic / pharmacology
Ribonucleases / genetics*,  metabolism*
Saccharomyces cerevisiae Proteins / genetics*,  metabolism*
Grant Support
ID/Acronym/Agency:
AI045995/AI/NIAID NIH HHS; AI049371/AI/NIAID NIH HHS; AI062124/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Antifungal Agents; 0/Echinocandins; 0/Mannose-Binding Lectin; 0/Peptides, Cyclic; 0/Saccharomyces cerevisiae Proteins; EC 3.1.-/CCR4 protein, S cerevisiae; EC 3.1.-/Ribonucleases; F0XDI6ZL63/caspofungin
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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