Document Detail

Binding of progesterone to cell surfaces of human granulosa-lutein cells.
MedLine Citation:
PMID:  16905308     Owner:  NLM     Status:  MEDLINE    
Progesterone is produced by granulosa cells under the influence of luteinizing hormone. Nuclear progesterone receptors have been found in rat granulosa cells. Human granulosa-lutein cells rapidly respond to progesterone with an increase in intracellular calcium suggesting the existence of a nongenomic mechanism. This study was conducted to determine whether binding of progesterone to granulosa cells could occur at the membrane. Granulosa cells were obtained from an in vitro fertilization program and examined immunohistochemically with an antiserum to membrane progesterone receptors. Approximately 14-70% of freshly harvested or cultured granulosa cells of six patients showed a positive reaction to the antiserum, limited to the cell membrane. Western blot analysis of homogenates of granulosa cells and a granulosa cell tumour confirmed the presence of progesterone receptors A, B and C and low amounts of a putative membrane receptor. These results demonstrate that the plasma membranes of human granulosa cells possess binding components for progesterone which may be involved in its nongenomic mechanism of action.
Edward V Younglai; Yanjing Wu; Warren G Foster; Derek K Lobb; Thomas M Price
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-08-14
Journal Detail:
Title:  The Journal of steroid biochemistry and molecular biology     Volume:  101     ISSN:  0960-0760     ISO Abbreviation:  J. Steroid Biochem. Mol. Biol.     Publication Date:  2006 Sep 
Date Detail:
Created Date:  2006-09-04     Completed Date:  2006-11-02     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9015483     Medline TA:  J Steroid Biochem Mol Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  61-7     Citation Subset:  IM    
Department of Obstetrics & Gynecology, Reproductive Biology Division, McMaster University, Health Sciences Centre, 1200 Main Street West, Hamilton, Ont., Canada L8N 3Z5.
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MeSH Terms
Binding Sites
Blotting, Western
Cell Membrane / metabolism
Granulosa Cells / metabolism*
Lutein / metabolism*
Progesterone / metabolism*
Reg. No./Substance:
127-40-2/Lutein; 57-83-0/Progesterone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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