Document Detail

Binding of phytochrome B to its nuclear signalling partner PIF3 is reversibly induced by light.
MedLine Citation:
PMID:  10466729     Owner:  NLM     Status:  MEDLINE    
The phytochrome photoreceptor family directs plant gene expression by switching between biologically inactive and active conformers in response to the sequential absorption of red and farred photons. Several intermediates that act late in the phytochrome signalling pathway have been identified, but fewer have been identified that act early in the pathway. We have cloned a nuclear basic helix-loop-helix protein, PIF3, which can bind to non-photoactive carboxy-terminal fragments of phytochromes A and B and functions in phytochrome signalling in vivo. Here we show that full-length photoactive phytochrome B binds PIF3 in vitro only upon light-induced conversion to its active form, and that photoconversion back to its inactive form causes dissociation from PIF3. We conclude that photosensory signalling by phytochrome B involves light-induced, conformer-specific recognition of the putative transcriptional regulator PIF3, providing a potential mechanism for direct photoregulation of gene expression.
M Ni; J M Tepperman; P H Quail
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Nature     Volume:  400     ISSN:  0028-0836     ISO Abbreviation:  Nature     Publication Date:  1999 Aug 
Date Detail:
Created Date:  1999-09-08     Completed Date:  1999-09-08     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0410462     Medline TA:  Nature     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  781-4     Citation Subset:  IM    
Department of Plant and Microbial Biology, University of California, Berkeley 94720, USA.
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MeSH Terms
Arabidopsis Proteins*
Basic Helix-Loop-Helix Transcription Factors
Binding Sites
Cloning, Molecular
Escherichia coli
Gene Expression Regulation, Plant / radiation effects
Photoreceptor Cells*
Phytochrome / genetics,  metabolism*
Phytochrome B
Protein Binding / radiation effects
Recombinant Fusion Proteins
Signal Transduction
Transcription Factors*
Reg. No./Substance:
0/Arabidopsis Proteins; 0/Basic Helix-Loop-Helix Transcription Factors; 0/PHYB protein, Arabidopsis; 0/PIF3 protein, Arabidopsis; 0/Recombinant Fusion Proteins; 0/Transcription Factors; 11121-56-5/Phytochrome; 136250-22-1/Phytochrome B
Comment In:
Nature. 1999 Aug 19;400(6746):710-1, 713   [PMID:  10466717 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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