| Binding-induced activation of overexpressed p185HER2 is essential in triggering neuronal differentiation of PC12 cells. | |
| | |
MedLine Citation:
|
PMID: 9361187 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
To determine whether p185HER2 overexpression per se triggers p185HER2 cellular signaling or whether an extracellular signal is required, we transfected PC12 cells with the human erbB-2 proto-oncogene, and established a cell line that overexpresses p185HER2. PC12-HER2 cells, maintained in suspension culture or plated on a collagen layer, showed the same morphology and growth rate as PC12 and PC12 mock-transfected control cells. When treated with monoclonal antibody (MAb) MGr6 or other anti-p185HER2 MAbs, PC12-HER2 cells specifically underwent neuronal differentiation comparable to that induced by nerve growth factor (NGF), and the differentiation-inducing effect of the MAb was dramatically enhanced by the addition of a second anti-mouse IgG. MAb-induced cell differentiation correlated with p185HER2 phosphorylation, recruitment of Shc and Grb-2 transducer molecules into complexes, and MAPK phosphorylation. These data indicate the requirement for a specific binding-induced activation of the overexpressed p185HER2 receptor in inducing PC12 cell differentiation. PC12-HER2 cells represent a suitable system for selection of p185HER2-activating ligands (peptides, phage-displayed peptides or proteins) or specific inhibitors of its tyrosine kinase activity. |
| | |
Authors:
|
R Orlandi; C Formantici; M I Colnaghi; S Ménard |
Related Documents
:
|
8074717 - Amyloid precursor protein secretion via muscarinic receptors: reduced desensitization u... 19061057 - Thirty-five percent oxygen pre-conditioning protects pc12 cells against death induced b... 1530637 - Vitamin k2 modulates proliferation and function of osteoblastic cells in vitro. 3595867 - Phagocytic cells metabolize 25-hydroxyvitamin d3 to 10-oxo-19-nor-25-hydroxyvitamin d3 ... 16513897 - Lipidomics of cellular and secreted phospholipids from differentiated human fetal type ... 10519047 - Cellular dynamics of corneal wound re-epithelialization in the rat. ii. dna synthesis o... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Journal of cellular biochemistry Volume: 67 ISSN: 0730-2312 ISO Abbreviation: J. Cell. Biochem. Publication Date: 1997 Dec |
Date Detail:
|
Created Date: 1997-12-10 Completed Date: 1997-12-10 Revised Date: 2009-11-19 |
Medline Journal Info:
|
Nlm Unique ID: 8205768 Medline TA: J Cell Biochem Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 316-26 Citation Subset: IM |
Affiliation:
|
Division of Experimental Oncology E, Istituto Nazionale per lo Studio e la Cura dei Tumori, Milan, Italy. Orlandi@istitutotumori.mi.it |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Antibodies, Monoclonal Calcium-Calmodulin-Dependent Protein Kinases / analysis Cell Differentiation Gene Expression Humans Mitogen-Activated Protein Kinase 1 / analysis Mitogen-Activated Protein Kinase 3 Mitogen-Activated Protein Kinases* Neurites Neurons / cytology* PC12 Cells Phosphotyrosine / analysis Rats Receptor, erbB-2 / biosynthesis, genetics, physiology* Signal Transduction Transfection |
| Chemical | |
Reg. No./Substance:
|
0/Antibodies, Monoclonal; 21820-51-9/Phosphotyrosine; EC 2.7.10.1/Receptor, erbB-2; EC 2.7.11.17/Calcium-Calmodulin-Dependent Protein Kinases; EC 2.7.11.24/Mitogen-Activated Protein Kinase 1; EC 2.7.11.24/Mitogen-Activated Protein Kinase 3; EC 2.7.11.24/Mitogen-Activated Protein Kinases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Identification of a cysteine protease responsible for degradation of sperm histones during male pron...
Next Document: Cyclic strain stimulates isoform-specific PKC activation and translocation in cultured human keratin...