| Binding of human hemoglobin by Haemophilus influenzae. | |
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MedLine Citation:
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PMID: 8020748 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Binding of biotinylated human hemoglobin to Haemophilus influenzae was detected when organisms were grown in heme-deplete, but not heme-replete, conditions. Hemoglobin binding was completely inhibited by a 100-fold excess of unlabelled human hemoglobin or human hemoglobin complexed with human haptoglobin. Binding was only partially inhibited by rat hemoglobin, bovine hemoglobin, human globin, and bovine globin, and not at all by heme, human serum albumin, bovine serum albumin, human transferrin, or myoglobin. Hemoglobin binding was saturable at 16-20 ng of hemoglobin per 10(9) cfu. Binding of human hemoglobin was detected in serotypes a-f and serologically non-typable strains of H. influenzae, as well as Haemophilus haemolyticus but not Haemophilus parainfluenzae, Haemophilus aphrophilus, Haemophilus parahaemolyticus, or Escherichia coli. |
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Authors:
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M E Frangipane; D J Morton; J A Wooten; J M Pozsgay; T L Stull |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: FEMS microbiology letters Volume: 118 ISSN: 0378-1097 ISO Abbreviation: FEMS Microbiol. Lett. Publication Date: 1994 May |
Date Detail:
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Created Date: 1994-08-03 Completed Date: 1994-08-03 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 7705721 Medline TA: FEMS Microbiol Lett Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 243-8 Citation Subset: IM |
Affiliation:
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Department of Pediatrics, Medical College of Pennsylvania, Philadelphia 19129. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Bacterial Outer Membrane Proteins / metabolism Cattle Haemophilus / classification, metabolism Haemophilus influenzae / growth & development, metabolism* Heme / metabolism Hemoglobins / metabolism* Humans Protein Binding Rats Species Specificity |
| Grant Support | |
ID/Acronym/Agency:
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AI29611/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Bacterial Outer Membrane Proteins; 0/Hemoglobins; 14875-96-8/Heme |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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