| Binding and functional characteristics of two E-box motifs within the S100A6 (calcyclin) gene promoter. | |
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MedLine Citation:
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PMID: 16288473 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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S100A6 (calcyclin) is a small calcium-binding protein of the S100 family often associated with cancer and metastasis. We have previously shown that the E-box sequence at position -283/-278 of the S100A6 gene promoter interacts with USF transcription factor and contributes to promoter transcriptional activity. We now present evidence that a second E-box motif at position -593/-588 of the promoter also binds USF and that the USF1/USF2 heterodimer is the prevailing dimeric form of the transcription factor bound. Using the chromatin immunoprecipitation assay (ChIP), we show that USF is bound in vivo to the E-box regulatory element(s). Depletion of the endogenous USF pool by means of a decoy oligonucleotide evokes a severe inhibition of S100A6 gene promoter activity. Furthermore, we show that S100A6 gene promoter activity can be stimulated by palmitate and that mutation of the -283/-278 E-box sequence completely blocks this stimulation. |
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Authors:
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Wiesława Leśniak; Jacek Kuźnicki |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of cellular biochemistry Volume: 97 ISSN: 0730-2312 ISO Abbreviation: J. Cell. Biochem. Publication Date: 2006 Apr |
Date Detail:
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Created Date: 2006-03-27 Completed Date: 2006-08-10 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 8205768 Medline TA: J Cell Biochem Country: United States |
Other Details:
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Languages: eng Pagination: 1017-24 Citation Subset: IM |
Copyright Information:
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(c) 2005 Wiley-Liss, Inc. |
Affiliation:
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Department of Molecular and Cellular Neurobiology, Nencki Institute of Experimental Biology, 3 Pasteur street, 02-093 Warsaw, Poland. w.lesniak@nencki.gov.pl |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Binding Sites Cell Cycle Proteins / genetics*, metabolism Cell Line DNA-Binding Proteins / metabolism E-Box Elements* Gene Expression Regulation Humans Mutation Palmitates / pharmacology Phosphorylation Promoter Regions, Genetic* Protein Binding S100 Proteins / genetics*, metabolism Transcription, Genetic* Upstream Stimulatory Factors / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Cell Cycle Proteins; 0/DNA-Binding Proteins; 0/Palmitates; 0/S100 Proteins; 0/USF1 protein, human; 0/Upstream Stimulatory Factors; 105504-00-5/S100A6 protein, human |
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