Document Detail


Binding of fatty acid ethyl esters to albumin for transport to cells in culture.
MedLine Citation:
PMID:  9989278     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Fatty acid ethyl esters (FAEE) are non-oxidative products of ethanol metabolism that have been proposed to mediate pathological changes in various organs and tissues resulting from excessive ethanol consumption. Evidence supporting this proposal is scant, however, mainly because of the lack of adequate methods with which to solubilize the highly hydrophobic FAEE in aqueous medium for testing under physiological conditions. In this report we describe a simple and practical method for solubilizing FAEE in aqueous medium by binding them to albumin. We also report that the albumin-bound FAEE are readily taken up by rat alveolar macrophages in culture. The availability of FAEE bound to albumin, their main physiological carrier in vivo, will facilitate the investigation of the role that these metabolites may have in mediating pathological changes associated with excess ethanol consumption.
Authors:
S Chang; J Borensztajn
Related Documents :
8043008 - Studies on the effect of an heterologous fatty acid-binding protein on acyl-coa oxidase...
7611458 - Metabolic effects of fatty acid-bearing albumin on a proximal tubule cell line.
17137378 - Synthesis of benzophenone-containing fatty acids.
16592818 - Localization of the amino acid substitution site in a new variant of human serum albumi...
960788 - Biological effects of transfatty acids.
14764908 - Lipoic acid-dependent oxidative catabolism of alpha-keto acids in mitochondria provides...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  1436     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  1999 Jan 
Date Detail:
Created Date:  1999-03-04     Completed Date:  1999-03-04     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  491-8     Citation Subset:  IM    
Affiliation:
Department of Pathology, Northwestern University Medical School, Chicago, IL 60611, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Albumins / metabolism*,  pharmacokinetics
Animals
Biological Transport, Active
Cells, Cultured
Ethanol / metabolism,  toxicity
Fatty Acids / metabolism*,  pharmacokinetics
Kinetics
Macrophages, Alveolar / metabolism
Oleic Acids / metabolism,  pharmacokinetics
Oxidation-Reduction
Protein Binding
Rats
Solubility
Water
Chemical
Reg. No./Substance:
0/Albumins; 0/Fatty Acids; 0/Oleic Acids; 111-62-6/ethyl oleate; 64-17-5/Ethanol; 7732-18-5/Water

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Reduced lecithin:retinol acyl transferase activity in cultured squamous cell carcinoma lines results...
Next Document:  Fasting increases tissue uptake and interconversion of plasma unesterified linoleic acid in guinea p...