Document Detail

Binding of fatty acid ethyl esters to albumin for transport to cells in culture.
MedLine Citation:
PMID:  9989278     Owner:  NLM     Status:  MEDLINE    
Fatty acid ethyl esters (FAEE) are non-oxidative products of ethanol metabolism that have been proposed to mediate pathological changes in various organs and tissues resulting from excessive ethanol consumption. Evidence supporting this proposal is scant, however, mainly because of the lack of adequate methods with which to solubilize the highly hydrophobic FAEE in aqueous medium for testing under physiological conditions. In this report we describe a simple and practical method for solubilizing FAEE in aqueous medium by binding them to albumin. We also report that the albumin-bound FAEE are readily taken up by rat alveolar macrophages in culture. The availability of FAEE bound to albumin, their main physiological carrier in vivo, will facilitate the investigation of the role that these metabolites may have in mediating pathological changes associated with excess ethanol consumption.
S Chang; J Borensztajn
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  1436     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  1999 Jan 
Date Detail:
Created Date:  1999-03-04     Completed Date:  1999-03-04     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  491-8     Citation Subset:  IM    
Department of Pathology, Northwestern University Medical School, Chicago, IL 60611, USA.
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MeSH Terms
Albumins / metabolism*,  pharmacokinetics
Biological Transport, Active
Cells, Cultured
Ethanol / metabolism,  toxicity
Fatty Acids / metabolism*,  pharmacokinetics
Macrophages, Alveolar / metabolism
Oleic Acids / metabolism,  pharmacokinetics
Protein Binding
Reg. No./Substance:
0/Albumins; 0/Fatty Acids; 0/Oleic Acids; 111-62-6/ethyl oleate; 64-17-5/Ethanol; 7732-18-5/Water

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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