Document Detail


Binding characteristics of various neurochemicals to glassy carbon.
MedLine Citation:
PMID:  8104952     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The determination of acetylcholine and choline using liquid chromatography with electrochemical detection using the approach of Potter et al. [J. Neurochem., 41 (1983) 188] normally requires isolation of the desired species before an analysis of tissue samples can be undertaken due to coeluting interferences afforded by other neutrochemicals. We have recently shown that this problem can be overcome by the use of a glassy carbon precolumn to effectively trap the interfering species [Ikarashi et al., J. Chromatogr., 575 (1992) 29]. We now report on the nature and mechanism of this adsorption onto glassy carbon for norepinephrine, dopamine, serotonin, 3,4-dihydroxyphenylacetic acid, homovanillic acid and 5-hydroxyindoleacetic acid. For both the acidic and basic compounds which comprise this group, distinct Langmuir adsorption processes appear to be involved for both the neutral and ionic forms of the individual compounds. Using various data fitting approaches, we have attempted to derive appropriate adsorption constants for the two forms of each compound. Theoretical predictions employing these derived constants provided results which match reasonably well with the observed adsorption data in most cases.
Authors:
Y Ikarashi; C L Blank; Y Maruyama
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of chromatography     Volume:  645     ISSN:  -     ISO Abbreviation:  J. Chromatogr.     Publication Date:  1993 Aug 
Date Detail:
Created Date:  1993-11-16     Completed Date:  1993-11-16     Revised Date:  2013-12-13    
Medline Journal Info:
Nlm Unique ID:  0427043     Medline TA:  J Chromatogr     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  219-31     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adsorption
Carbon / chemistry
Chromatography, High Pressure Liquid
Electrochemistry
Hydrogen-Ion Concentration
Indicators and Reagents
Neurotransmitter Agents / chemistry*
Grant Support
ID/Acronym/Agency:
GM32367/GM/NIGMS NIH HHS; NCRR 2S07 RR070778-18/RR/NCRR NIH HHS
Chemical
Reg. No./Substance:
0/Indicators and Reagents; 0/Neurotransmitter Agents; 7440-44-0/Carbon

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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