Document Detail


Binding characteristics of recombinant human endothelin receptors ETA and ETB expressed in baby hamster kidney cells.
MedLine Citation:
PMID:  7509929     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Expression constructs for human endothelin (ET) receptors ETA and ETB were made by subcloning the corresponding coding sequences into the pMPSV/CMV vector. They were used together with plasmids bearing resistances to puromycin and hygromycin for transfection of baby hamster kidney cells. Culture in the presence of both antibiotics allowed the selection of cell lines stably expressing one receptor or the other. Competitive binding experiments using ET-1 and ET-3 showed the typical isopeptide-selective and non-isopeptide-selective profiles for recombinant ETA and ETB, respectively.
Authors:
U Hechler; A Becker; B Haendler; W D Schleuning
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of cardiovascular pharmacology     Volume:  22 Suppl 8     ISSN:  0160-2446     ISO Abbreviation:  J. Cardiovasc. Pharmacol.     Publication Date:  1993  
Date Detail:
Created Date:  1994-04-04     Completed Date:  1994-04-04     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  7902492     Medline TA:  J Cardiovasc Pharmacol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  S15-7     Citation Subset:  IM    
Affiliation:
Research Laboratories of Schering AG, Berlin, Germany.
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MeSH Terms
Descriptor/Qualifier:
Animals
Binding, Competitive / drug effects
Cell Line
Cinnamates*
Cricetinae
Eukaryotic Cells / drug effects,  metabolism
Humans
Hygromycin B / analogs & derivatives,  pharmacology
Iodine Radioisotopes / diagnostic use
Kidney / metabolism
Puromycin / pharmacology
Receptors, Endothelin / metabolism*
Recombinant Proteins / metabolism
Transfection
Chemical
Reg. No./Substance:
0/Cinnamates; 0/Iodine Radioisotopes; 0/Receptors, Endothelin; 0/Recombinant Proteins; 31282-04-9/Hygromycin B; 53-79-2/Puromycin; 6379-56-2/hygromycin A

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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