| Binding characteristics of recombinant human endothelin receptors ETA and ETB expressed in baby hamster kidney cells. | |
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MedLine Citation:
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PMID: 7509929 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Expression constructs for human endothelin (ET) receptors ETA and ETB were made by subcloning the corresponding coding sequences into the pMPSV/CMV vector. They were used together with plasmids bearing resistances to puromycin and hygromycin for transfection of baby hamster kidney cells. Culture in the presence of both antibiotics allowed the selection of cell lines stably expressing one receptor or the other. Competitive binding experiments using ET-1 and ET-3 showed the typical isopeptide-selective and non-isopeptide-selective profiles for recombinant ETA and ETB, respectively. |
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Authors:
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U Hechler; A Becker; B Haendler; W D Schleuning |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of cardiovascular pharmacology Volume: 22 Suppl 8 ISSN: 0160-2446 ISO Abbreviation: J. Cardiovasc. Pharmacol. Publication Date: 1993 |
Date Detail:
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Created Date: 1994-04-04 Completed Date: 1994-04-04 Revised Date: 2005-11-17 |
Medline Journal Info:
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Nlm Unique ID: 7902492 Medline TA: J Cardiovasc Pharmacol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: S15-7 Citation Subset: IM |
Affiliation:
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Research Laboratories of Schering AG, Berlin, Germany. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Binding, Competitive / drug effects Cell Line Cinnamates* Cricetinae Eukaryotic Cells / drug effects, metabolism Humans Hygromycin B / analogs & derivatives, pharmacology Iodine Radioisotopes / diagnostic use Kidney / metabolism Puromycin / pharmacology Receptors, Endothelin / metabolism* Recombinant Proteins / metabolism Transfection |
| Chemical | |
Reg. No./Substance:
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0/Cinnamates; 0/Iodine Radioisotopes; 0/Receptors, Endothelin; 0/Recombinant Proteins; 31282-04-9/Hygromycin B; 53-79-2/Puromycin; 6379-56-2/hygromycin A |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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