| Binding characteristics of human serum amyloid P component. | |
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MedLine Citation:
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PMID: 3105045 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Serum amyloid P component (SAP), a normal human plasma glycoprotein, was found in a solid phase ELISA to have Ca2+-dependent binding for keyhole limpet haemocyanin (KLH), pectic acid, trinitrophenylated (TNP) macromolecules, and plastic surfaces. The binding to TNP-KLH was used to develop a sensitive ELISA. The binding of SAP to the ligands mentioned was inhibited by EDTA, KLH, pectic acid, TNP-conjugated macromolecules (bovine serum albumin, polyacrylhydrazide), and p-nitrophenylarsonic acid. Underivatized and DNP-conjugated macromolecules did not inhibit the SAP binding; arsenilic acid, picric acid, and dinitrophenyl were weak inhibitors. SAP bound to TNP-agarose was eluated by either EDTA or p-nitrophenylarsonic acid. Thus, a unique region of SAP is responsible for the polyspecific binding. We suggest that the polyspecific binding of SAP takes place through a Ca2+ bridge: half of the metal coordination sphere is occupied by SAP, with the other half available to interact with metal ligand. |
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Authors:
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D Serban; C Rordorf-Adam |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Scandinavian journal of immunology Volume: 25 ISSN: 0300-9475 ISO Abbreviation: Scand. J. Immunol. Publication Date: 1987 Mar |
Date Detail:
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Created Date: 1987-05-11 Completed Date: 1987-05-11 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 0323767 Medline TA: Scand J Immunol Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 275-81 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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C-Reactive Protein
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metabolism Calcium / pharmacology Chromatography, Gel Edetic Acid / pharmacology Enzyme-Linked Immunosorbent Assay / methods Hemocyanin / metabolism Humans Pectins / metabolism Plastics / metabolism Protein Binding Serum Albumin, Bovine / metabolism Serum Amyloid P-Component / isolation & purification, metabolism* Surface Properties Trinitrobenzenes / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Pectins; 0/Plastics; 0/Serum Albumin, Bovine; 0/Serum Amyloid P-Component; 0/Trinitrobenzenes; 0/keyhole-limpet hemocyanin; 60-00-4/Edetic Acid; 7440-70-2/Calcium; 9007-41-4/C-Reactive Protein; 9013-72-3/Hemocyanin; 9046-38-2/polygalacturonic acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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