| Binding of bile acids, oleic acid, and organic anions by rat and human hepatic Z protein. | |
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MedLine Citation:
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PMID: 3789743 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Binding affinities of purified Z proteins from rat and human liver for bile acids, oleic acid, and organic anions were studied. Purification of Z protein from both rat and human hepatic cytosol was performed by gel filtration, chromatofocusing, and hydroxyapatite chromatography. Both purified proteins showed the same molecular weight (Mr = 14,000) and isoelectric points were 6.9 and 6.5 for rat and human proteins, respectively. Binding studies were performed by the competitive displacement of 1-anilino-8-naphthalene sulfonate. Rat and human Z proteins exhibited similar binding affinities for bile acids, oleic acid, and organic anions. Among various bile acids, both proteins bound monohydroxy bile acids with high affinity and trihydroxy bile acids with low affinity; sulfates were bound with higher and glucuronides with lower affinity than their parent bile acids. In comparison with GSH S-transferases, rat Z protein had lower affinity for bile acids than rat GSH S-transferase B and human Z protein had higher affinity for bile acids than human cationic GSH S-transferase. The role for Z protein in the intracellular binding of bile acids may be particularly important in human liver. |
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Authors:
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H Takikawa; N Kaplowitz |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Archives of biochemistry and biophysics Volume: 251 ISSN: 0003-9861 ISO Abbreviation: Arch. Biochem. Biophys. Publication Date: 1986 Nov |
Date Detail:
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Created Date: 1987-01-06 Completed Date: 1987-01-06 Revised Date: 2005-11-17 |
Medline Journal Info:
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Nlm Unique ID: 0372430 Medline TA: Arch Biochem Biophys Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 385-92 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Anions / metabolism Bile Acids and Salts / metabolism* Carrier Proteins / isolation & purification, metabolism* Fatty Acid-Binding Proteins Glutathione Transferase / metabolism Humans Kinetics Ligands Liver / metabolism* Oleic Acid Oleic Acids / metabolism* Rats |
| Chemical | |
Reg. No./Substance:
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0/Anions; 0/Bile Acids and Salts; 0/Carrier Proteins; 0/Fatty Acid-Binding Proteins; 0/Ligands; 0/Oleic Acids; 112-80-1/Oleic Acid; EC 2.5.1.18/Glutathione Transferase |
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