| Binding assay for cholera toxin based on sequestration electrochemistry using lactose labeled with an electroactive compound. | |
| | |
MedLine Citation:
|
PMID: 21491033 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
A simple electrochemical binding assay for cholera toxin (CT) was developed using lactose labeled with daunomycin as an electroactive compound. The labeled lactose (LL) was determined with high sensitivity by adsorptive stripping voltammetry (AdSV). The electrochemical behaviors of LL at glassy carbon (GC), plastic formed carbon (PFC) and carbon nanotubes paste (CNTP) electrode were investigated. The CNTP electrode showed the greatest accumulation capacity for LL. The assay for CT based on the sequestration electrochemistry was demonstrated. The binding event of the LL to CT was detected by the decrease in the electrochemical response of daunomycin as an electroactive label without a separation process to remove the free LL from the one bound with CT before any measurements can be made. The detection limit of the CT assay using the CNTP electrode was 0.5 nM (42 ng mL(-1)). |
| | |
Authors:
|
Hideki Kuramitz; Shun Miyagaki; Eiji Ueno; Noriko Hata; Shigeru Taguchi; Kazuharu Sugawara |
Related Documents
:
|
21308473 - Does lateral vertebral translation correspond to cobb angle and relate in the same way ... 1902033 - Hamartomas of the tuber cinereum: ct, mr, and pathologic findings. 16520993 - Lumbar spine mri in the elite-level female gymnast with low back pain. |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2011-4-14 |
Journal Detail:
|
Title: The Analyst Volume: - ISSN: 1364-5528 ISO Abbreviation: - Publication Date: 2011 Apr |
Date Detail:
|
Created Date: 2011-4-14 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0372652 Medline TA: Analyst Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
|
Department of Environmental Biology and Chemistry, Graduate School of Science and Engineering for Research, University of Toyama, Gofuku 3190, Toyama, 930-8555, Japan. kuramitz@sci.u-toyama.ac.jp. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Exciton diffusion and charge transfer dynamics in nano phase-separated P3HT/PCBM blend films.
Next Document: A facile method to tune zeolite L crystals with low aspect ratio.