| Binding affinities of commonly employed sensitizers of viral inactivation. | |
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MedLine Citation:
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PMID: 12081315 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Methylene blue (MB), riboflavin (RB) and psoralen sensitizers (4' aminomethyl-4,5',8-trimethylpsoralen [AMT] and derivatives) are under study as sensitizers of viral inactivation of blood products such as plasma proteins, platelets and red cells, all of which lack genomic nucleic acid. To predict where these sensitizers accumulate in viruses and in cells, their relative affinities for calf thymus DNA, neutral and negatively charged phospholipids and albumin were determined by dialysis. MB has a strong affinity for nucleic acid and negatively charged phospholipid, but little affinity for albumin or neutral phospholipid. RB has modest affinity for nucleic acid and little affinity for albumin or either phospholipid. AMT has substantial affinity for nucleic acid, neutral and negatively charged phospholipids and albumin. Neither AMT nor RB binds to poly G, although MB has some affinity for this polymer. Evidence of association of RB with guanosine monophosphate, adenosine monophosphate and tryptophan methylester hydrochloride in PBS buffer in the presence and absence of formamide was obtained from nonlinear Stern-Volmer plots and shifts in the ground state absorption spectrum of RB. |
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Authors:
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Nathalie Dardare; Matthew S Platz |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Photochemistry and photobiology Volume: 75 ISSN: 0031-8655 ISO Abbreviation: Photochem. Photobiol. Publication Date: 2002 Jun |
Date Detail:
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Created Date: 2002-06-25 Completed Date: 2002-08-02 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0376425 Medline TA: Photochem Photobiol Country: United States |
Other Details:
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Languages: eng Pagination: 561-4 Citation Subset: IM |
Affiliation:
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Department of Chemistry, The Ohio State University, Columbus, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Photosensitizing Agents
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pharmacology* Trioxsalen / analogs & derivatives*, pharmacology* Virus Activation / drug effects* Viruses / drug effects, growth & development* |
| Chemical | |
Reg. No./Substance:
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0/Photosensitizing Agents; 3902-71-4/Trioxsalen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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