Document Detail


Binding of 8-anilinonaphthalene sulfonate to dimeric and tetrameric concanavalin A: energetics and its implications on saccharide binding studied by isothermal titration calorimetry and spectroscopy.
MedLine Citation:
PMID:  16571017     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The binding of 8-anilinonaphthalene sulfonate to concanavalin A has been investigated. Isothermal titration calorimetry (ITC) and circular dichroism studies have been performed under different experimental conditions to understand the binding quantitatively and evaluate contributions of different forces responsible for it. Isothermal titration calorimetric results of concanavalin A with ANS at pH 5.2 and 2.5, where it exists as a dimer, indicated binding heterogeneity and two classes of noninteracting sites. Enhancement of the binding constants from native to pH 2.5 suggests that the ANS binding is strongly influenced by the protein charge and the favorable alteration in the structure of concanavalin A as suggested by near-UV CD results. No binding was observed with the tetrameric form of concanavalin A, indicating shielding of sites due to dimerization of canonical dimers. The results have also demonstrated existence of a hydrophobic binding site that is distinct from the saccharide binding site.
Authors:
Tuhina Banerjee; Nand Kishore
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The journal of physical chemistry. B     Volume:  110     ISSN:  1520-6106     ISO Abbreviation:  J Phys Chem B     Publication Date:  2006 Apr 
Date Detail:
Created Date:  2006-03-30     Completed Date:  2007-07-31     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  101157530     Medline TA:  J Phys Chem B     Country:  United States    
Other Details:
Languages:  eng     Pagination:  7022-8     Citation Subset:  IM    
Affiliation:
Department of Chemistry, Indian Institute of Technology, Bombay, Powai, Mumbai 400 076, India.
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MeSH Terms
Descriptor/Qualifier:
Anilino Naphthalenesulfonates / chemistry*
Calorimetry
Chemistry, Physical
Circular Dichroism
Concanavalin A / chemistry*
Hydrogen-Ion Concentration
Maltose / chemistry*
Physicochemical Phenomena
Protein Conformation
Temperature
Titrimetry
Chemical
Reg. No./Substance:
0/Anilino Naphthalenesulfonates; 11028-71-0/Concanavalin A; 69-79-4/Maltose; 82-76-8/1-anilino-8-naphthalenesulfonate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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