| Binding of [3H]benzo(a)pyrene metabolites to the AKR mouse embryo cell line nuclear proteins. | |
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MedLine Citation:
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PMID: 6260346 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Nuclear proteins from AKR-2B mouse embryo cells incubated 24 hr with [3H]benzo(a)pyrene (1 microgram/ml; 10 microCi/ml) were purified to remove nucleic acids and characterized by isoelectric focusing and sodium dodecyl sulfate gel electrophoresis on polyacrylamide gels. Analysis of the nuclear proteins by isoelectric focusing revealed a distribution of protein in the pH gradient with two prominent bands of proteins, one focusing at pH 5 and the other at pH 11, both of which bound significant amounts of the carcinogen. The acid-soluble proteins from the nuclear preparation were analyzed by isoelectric focusing, and the results also revealed two peaks of protein and radioactivity at pH's of 5 and 11. Further analysis of the acid-soluble fraction on acid urea gels revealed that significant radioactivity was associated with proteins that migrated near but slower than histone H1 which may be the high-mobility group proteins 1 and 2. Radioactivity also comigrated with histones H3 and H2B. Low levels of radioactivity were associated with histone H1 and H4. |
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Authors:
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T H Zytkovicz; H L Moses; T C Spelsberg |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Cancer research Volume: 41 ISSN: 0008-5472 ISO Abbreviation: Cancer Res. Publication Date: 1981 May |
Date Detail:
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Created Date: 1981-06-25 Completed Date: 1981-06-25 Revised Date: 2003-11-14 |
Medline Journal Info:
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Nlm Unique ID: 2984705R Medline TA: Cancer Res Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1608-14 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Benzo(a)pyrene Benzopyrenes / metabolism* Cell Line Cell Nucleus / metabolism* Chromosomal Proteins, Non-Histone / metabolism* High Mobility Group Proteins Histones / metabolism* Isoelectric Point Mice Phosphorylation Protein Binding |
| Chemical | |
Reg. No./Substance:
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0/Benzopyrenes; 0/Chromosomal Proteins, Non-Histone; 0/High Mobility Group Proteins; 0/Histones; 50-32-8/Benzo(a)pyrene |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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