Document Detail


Binding of [3H]benzo(a)pyrene metabolites to the AKR mouse embryo cell line nuclear proteins.
MedLine Citation:
PMID:  6260346     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Nuclear proteins from AKR-2B mouse embryo cells incubated 24 hr with [3H]benzo(a)pyrene (1 microgram/ml; 10 microCi/ml) were purified to remove nucleic acids and characterized by isoelectric focusing and sodium dodecyl sulfate gel electrophoresis on polyacrylamide gels. Analysis of the nuclear proteins by isoelectric focusing revealed a distribution of protein in the pH gradient with two prominent bands of proteins, one focusing at pH 5 and the other at pH 11, both of which bound significant amounts of the carcinogen. The acid-soluble proteins from the nuclear preparation were analyzed by isoelectric focusing, and the results also revealed two peaks of protein and radioactivity at pH's of 5 and 11. Further analysis of the acid-soluble fraction on acid urea gels revealed that significant radioactivity was associated with proteins that migrated near but slower than histone H1 which may be the high-mobility group proteins 1 and 2. Radioactivity also comigrated with histones H3 and H2B. Low levels of radioactivity were associated with histone H1 and H4.
Authors:
T H Zytkovicz; H L Moses; T C Spelsberg
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Cancer research     Volume:  41     ISSN:  0008-5472     ISO Abbreviation:  Cancer Res.     Publication Date:  1981 May 
Date Detail:
Created Date:  1981-06-25     Completed Date:  1981-06-25     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  2984705R     Medline TA:  Cancer Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1608-14     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Benzo(a)pyrene
Benzopyrenes / metabolism*
Cell Line
Cell Nucleus / metabolism*
Chromosomal Proteins, Non-Histone / metabolism*
High Mobility Group Proteins
Histones / metabolism*
Isoelectric Point
Mice
Phosphorylation
Protein Binding
Chemical
Reg. No./Substance:
0/Benzopyrenes; 0/Chromosomal Proteins, Non-Histone; 0/High Mobility Group Proteins; 0/Histones; 50-32-8/Benzo(a)pyrene

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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