| Binding of [3H]-4,5-secodihydrotestosterone by androgen receptor. | |
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MedLine Citation:
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PMID: 4092742 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Specific binding of [5,6-3H2]-17 beta-hydroxy-4,5-secoandrostan-3-one (or [3H]-4,5-secodihydrotestosterone, IX) to rat prostate cytosol receptors was observed and confirmed by two independent methods: by adsorption to dextran-charcoal and by polyacrylamide gel electrophoresis. The electrophoresis of [3H]-4,5-secodihydrotestosterone--cytosol mixtures showed a single radio-activity peak at different radioligand concentrations, displaceable by nonradioactive 4,5-secodihydrotestosterone; the mobility of the peak corresponds to a protein with a relative molecular weight of 90 000-130 000. The apparent association constant of the compound with respect to adsorption to dextran-charcoal is 1.48 X 10(9) l X mol-1, similar to the value observed with dihydrotestosterone (4.8 X 10(8) l X mol-1). |
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Authors:
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A Kasal; L Stárka; R Hampl; M Bicíková |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Experimental and clinical endocrinology Volume: 86 ISSN: 0232-7384 ISO Abbreviation: Exp. Clin. Endocrinol. Publication Date: 1985 Dec |
Date Detail:
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Created Date: 1986-04-04 Completed Date: 1986-04-04 Revised Date: 2003-11-14 |
Medline Journal Info:
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Nlm Unique ID: 8302802 Medline TA: Exp Clin Endocrinol Country: GERMANY, EAST |
Other Details:
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Languages: eng Pagination: 297-9 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cytosol / metabolism Dihydrotestosterone / analogs & derivatives*, metabolism Electrophoresis, Polyacrylamide Gel Male Prostate / metabolism* Rats Receptors, Androgen / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Receptors, Androgen; 521-18-6/Dihydrotestosterone; 76760-84-4/4,5-secodihydrotestosterone |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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