Document Detail


Binding of [3H]-4,5-secodihydrotestosterone by androgen receptor.
MedLine Citation:
PMID:  4092742     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Specific binding of [5,6-3H2]-17 beta-hydroxy-4,5-secoandrostan-3-one (or [3H]-4,5-secodihydrotestosterone, IX) to rat prostate cytosol receptors was observed and confirmed by two independent methods: by adsorption to dextran-charcoal and by polyacrylamide gel electrophoresis. The electrophoresis of [3H]-4,5-secodihydrotestosterone--cytosol mixtures showed a single radio-activity peak at different radioligand concentrations, displaceable by nonradioactive 4,5-secodihydrotestosterone; the mobility of the peak corresponds to a protein with a relative molecular weight of 90 000-130 000. The apparent association constant of the compound with respect to adsorption to dextran-charcoal is 1.48 X 10(9) l X mol-1, similar to the value observed with dihydrotestosterone (4.8 X 10(8) l X mol-1).
Authors:
A Kasal; L Stárka; R Hampl; M Bicíková
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Experimental and clinical endocrinology     Volume:  86     ISSN:  0232-7384     ISO Abbreviation:  Exp. Clin. Endocrinol.     Publication Date:  1985 Dec 
Date Detail:
Created Date:  1986-04-04     Completed Date:  1986-04-04     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  8302802     Medline TA:  Exp Clin Endocrinol     Country:  GERMANY, EAST    
Other Details:
Languages:  eng     Pagination:  297-9     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cytosol / metabolism
Dihydrotestosterone / analogs & derivatives*,  metabolism
Electrophoresis, Polyacrylamide Gel
Male
Prostate / metabolism*
Rats
Receptors, Androgen / metabolism*
Chemical
Reg. No./Substance:
0/Receptors, Androgen; 521-18-6/Dihydrotestosterone; 76760-84-4/4,5-secodihydrotestosterone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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