Document Detail


Binding of 131I-labeled tissue-type plasminogen activator on de-endothelialized lesions in rabbits.
MedLine Citation:
PMID:  3124079     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Tissue-type plasminogen activator (t-PA) which has a high affinity for fibrin in the clot, was labeled with 131I by the iodogen method, and its binding to de-endothelialized lesions in the rabbit was measured to assess the detectability of thrombi. The de-endothelialized lesion was induced in the abdominal aorta with a Fogarty 4F balloon catheter. Two hours after the de-endothelialization, 131I-labeled t-PA (125 +/- 46 microCi) was injected intravenously. The initial half-life of the agent in blood (n = 12) was 2.9 +/- 0.4 min. The degree of binding of 131I-labeled t-PA to the de-endothelialized lesion was evaluated at 15 min (n = 6) or at 30 min (n = 6) after injection of the agent. In spite of the retention of the biochemical properties of 131I-labeled t-PA and the presence of fibrin deposition at the de-endothelialized lesion, the binding of t-PA to the lesion was not sufficiently strong. Lesion-to-control ratios (cpm/g/cpm/g) were 1.65 +/- 0.40 (at 15 min) and 1.39 +/- 1.31 (at 30 min), and lesion-to-blood ratios were 1.39 +/- 0.32 (at 15 min) and 1.36 +/- 0.23 (at 30 min). These results suggest that radiolabeled t-PA may be inappropriate as a radiopharmaceutical for the scintigraphic detection of a pre-existing thrombotic lesion.
Authors:
A Uehara; Y Isaka; H Etani; K Kimura; S Yoneda; T Kamada; T Kozuka; M Nobuhara
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Nuklearmedizin. Nuclear medicine     Volume:  26     ISSN:  0029-5566     ISO Abbreviation:  Nuklearmedizin     Publication Date:  1987 Oct 
Date Detail:
Created Date:  1988-03-15     Completed Date:  1988-03-15     Revised Date:  2007-01-12    
Medline Journal Info:
Nlm Unique ID:  7609387     Medline TA:  Nuklearmedizin     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  224-8     Citation Subset:  IM    
Affiliation:
Division of Nuclear Medicine, Osaka University Medical School, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Fibrin / metabolism*
Iodine Radioisotopes / diagnostic use*
Male
Rabbits
Thrombosis / radionuclide imaging*
Tissue Plasminogen Activator / diagnostic use,  metabolism*
Chemical
Reg. No./Substance:
0/Iodine Radioisotopes; 9001-31-4/Fibrin; EC 3.4.21.68/Tissue Plasminogen Activator

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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